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Title

A commercial laccase-mediator system to delignify and improve saccharification of the fast-growing Paulownia fortunei (Seem.) Hemsl.

AuthorsRencoret, Jorge ; Pereira González, Antonio ; Marques, Gisela ; Río Andrade, José Carlos del ; Martínez, Ángel T. ; Gutiérrez Suárez, Ana
Keywords2D-NMR
Laccase
Lignin
Paulownia
Pretreatment
Saccharification
Issue DateJan-2019
PublisherWalter de Gruyter
CitationHolzforschung 73(1): 45–54 (2019)
AbstractIt was demonstrated for the first time that a laccase-based enzymatic pretreatment is able to delignify fast-growing paulownia species. The treatment was performed with a commercial low-redox potential laccase isolated from Myceliophthora thermophila (Apinis) Oorschot and methyl syringate (MeS) as a natural phenolic mediator. Up to 24% lignin removal was attained by the laccase-MeS treatment (L/MeS), followed by alkaline peroxide extraction in a multistage sequence. The reduction in lignin content was accompanied by a significant improvement in the subsequent enzymatic saccharification, with increases of up to 38% glucose and 34% xylose yields. The structural modifications of the lignin were analyzed in situ by two dimensional-nuclear magnetic resonance (2D-NMR) spectroscopy. A considerable removal of guaiacyl and syringyl lignin units with respect to the carbohydrate signals was visible as well as the cleavage of β-O-4′, β-5′ and β-β′ linkages leading to elevated amounts of Cα-oxidized guaiacyl and syringyl units. The presence of oxidized lignin compounds in the filtrates of the enzymatic treatments - such as vanillin, vanillic acid, syringaldehyde and syringic acid - conclusively demonstrates the ability of L/MeS treatment to oxidize and depolymerize the lignin in paulownia wood
Description10 páginas.-- 5 figuras.-- 3 tablas.-- 41 referencias.-- El archivo adjunto no es la última versión postprint
Publisher version (URL)http://dx.doi.org/10.1515/hf-2018-0095
URIhttp://hdl.handle.net/10261/172073
DOI10.1515/hf-2018-0095
ISSN0018-3830
E-ISSN1437-434X
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