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Respiratory analysis of intact Anisakis L3 as physiological tool to identify subtle changes in larvae subjected to thermal and/or chemical stress and still considered viable

AuthorsSánchez Alonso, Isabel ; Navas, Alfonso ; Arcos, Susana C.; González Muñoz, Miguel; Carballeda-Sangiao, Noelia; Careche, Mercedes
Issue Date2018
Citation48th West European Fish Technologists Association Meeting (2018)
AbstractHuman infection due to eating fish parasitized by live Anisakis larvae in the third stage (L3) is considered an important health problem and the application of treatments to ensure their mortality is crucial to prevent the risk of infection. Mobility is used as the method to assess viability. However, mobile larvae may not always be infective, and there is recognised a need to establish other methods to assess whether these larvae are capable of infecting humans. We suggest that mitochondria may become dysfunctional owing to various physical or chemical treatments applied to inactivate Anisakis L3, even if larvae survive these treatments and the oxygen consumption rate (OCR) could give valuable information about the actual mitochondrial function. We aimed to establish whether respiratory analysis of Anisakis L3 could identify differences between larvae considered viable, but that had been subjected to stress (thermal and/or chemical). The modulators FCCP and azide were used to obtain the basal, maximum, spare and residual respiration rates. The respiration analysis of larvae subjected to a certain temperature or environmental stress, (i.e. storage at 37 °C or in gastric juice), showed that mitochondria were affected compared to the untreated controls. The maximum respiratory capacity of larvae subjected to freezing could initially decrease immediately after thawing, but after some acclimatization they were able to recover their respiratory capacity fully. However, when treated larvae were stored at refrigeration temperatures their mitochondria became dysfunctional faster than those of untreated larvae. To conclude, Anisakis L3 responds to mitochondrial respiration modulators, so respiration analysis can be incorporated for in vivo assessment of mitochondrial function in this nematode. These measurements can be used as a tool to characterize L3 subjected to different stresses which, together with other indicators, help to give a broader picture of Anisakis L3 characteristics and potential infectivity.
DescriptionTrabajo presentado al 48th West European FishTechnologists Association Meeting (WEFTA), celebrado en Lisboa (Portugal) del 15 al 18 de octubre de 2018.
Appears in Collections:(ICTAN) Comunicaciones congresos
(MNCN) Comunicaciones congresos
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