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dc.contributor.authorEstevinho, Berta N.es_ES
dc.contributor.authorSamaniego, Nuriaes_ES
dc.contributor.authorTalens Perales, Davides_ES
dc.contributor.authorFabra, María Josées_ES
dc.contributor.authorLópez-Rubio, Amparoes_ES
dc.contributor.authorPolaina Molina, Julioes_ES
dc.contributor.authorMarín Navarro, Juliaes_ES
dc.date.accessioned2018-10-30T12:36:29Z-
dc.date.available2018-10-30T12:36:29Z-
dc.date.issued2018-04-18-
dc.identifier.citationInternational Journal of Biological Macromolecules 115: 476-482 (2018)es_ES
dc.identifier.issn0141-8130-
dc.identifier.urihttp://hdl.handle.net/10261/171743-
dc.description.abstractEnzymatically-active bacterial cellulose (BC) was prepared by non-covalent immobilization of a hybrid enzyme composed by a β-galactosidase from Thermotoga maritima (TmLac) and a carbohydrate binding module (CBM2) from Pyrococcus furiosus. TmLac-CBM2 protein was bound to BC, with higher affinity at pH 6.5 than at pH 8.5 and with high specificity compared to the non-engineered enzyme. Both hydrated (HBC) and freeze-dried (DBC) bacterial cellulose showed equivalent enzyme binding efficiencies. Initial reaction rate of HBC-bound enzyme was higher than DBC-bound and both of them were lower than the free enzyme. However, enzyme performance was similar in all three cases for the hydrolysis of 5% lactose to a high extent. Reuse of the immobilized enzyme was limited by the stability of the β-galactosidase module, whereas the CBM2 module provided stable attachment of the hybrid enzyme to the BC support, after long incubation periods (3 h) at 75 °C.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy, Industry and Competitiveness (grant number AGL2016-75245-R). Berta N. Estevinho thanks Fundação para a Ciência e a Tecnologia (FCT), Portugal, for postdoctoral grant SFRH/BPD/73865/2010. M.J. Fabra is recipient of a Ramon y Cajal (RYC-2014-158) contract from the Spanish Ministry of Economy, Industry and Competitiveness.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/AGL2016-75245-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectProtein immobilizationes_ES
dc.subjectCarbohydrate binding modulees_ES
dc.subjectBacterial cellulosees_ES
dc.titleDevelopment of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered β-galactosidasees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.ijbiomac.2018.04.081-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.ijbiomac.2018.04.081es_ES
dc.embargo.terms2019-04-18es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderFundação para a Ciência e a Tecnologia (Portugal)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001871es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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