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dc.contributor.authorMartínez-Periñán, E.es_ES
dc.contributor.authorSánchez-Tirado, E.es_ES
dc.contributor.authorGonzález-Cortés, Aracelies_ES
dc.contributor.authorBarderas, Rodrigoes_ES
dc.contributor.authorSánchez-Puelles, José Maríaes_ES
dc.contributor.authorMartínez-Santamaría, L.es_ES
dc.contributor.authorCampuzano, Susanaes_ES
dc.contributor.authorYáñez-Sedeño, Pilares_ES
dc.contributor.authorPingarrón, J.M.es_ES
dc.date.accessioned2018-10-19T09:20:08Z-
dc.date.available2018-10-19T09:20:08Z-
dc.date.issued2018-12-01-
dc.identifier.citationElectrochimica Acta 292: 887-894 (2018)es_ES
dc.identifier.issn0013-4686-
dc.identifier.urihttp://hdl.handle.net/10261/171261-
dc.description24 p.-6 fig.-2 tab.es_ES
dc.description.abstractAn amperometric immunosensor for the determination of the biomarker endoglin (CD105) to comply with the requirements of sensitivity and accuracy demanded in clinical practice is reported in this work. The immunosensing platform is implemented onto disposable electrodes modified with poly(pyrrolepropionic) acid (pPPA). The methodology involves a sandwich configuration and labeling of the biotinylated detector antibody with poly-HRP-streptavidin for signal amplification. Amperometric detection of hydrogen peroxide reduction in the presence of HQ was employed as analytical readout. The different steps involved in the immunosensor preparation were monitored by electrochemical impedance spectroscopy. The resulting immunosensor provided a linear range between 0.18 and 20 ng mL-1, adequate for the determination of CD105 in serum, with a detection limit (LOD) of 140 pg mL−1. These analytical characteristics improve those reported previously for other electrochemical immunosensors. A comparison with immunosensors prepared using other electrochemical scaffolds for anti-CD105 immobilization involving carboxylated doubled walled carbon nanotubes or modification by grafting, showed that a better performance was achieved using pPPA-modified SPCEs. A good reproducibility of the measurements, an excellent storage stability of the anti-CD105-pPPA/SPCE bioplatforms and an excellent selectivity of the resulting immunosensors were found. The usefulness of the immunosensors was tested by analyzing human serum samples collected from healthy individuals and patients of colorectal, breast and lung cancer and epidermolysis bullosa. The results were successfully validated against those provided by an ELISA kit.es_ES
dc.description.sponsorshipThe financial support of the Spanish Ministerio de Economía y Competitividad CTQ2015-70023-R and CTQ2015-64402-C2-1-R, and SAF2014-53209-R Research Projects, the PI17CIII/00045 research project from AESI and the NANOAVANSENS Program from the Comunidad de Madrid (S2013/MT-3029).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/CTQ2015-70023-Res_ES
dc.relationMINECO/ICTI2013-2016/CTQ2015-64402-C2-1-Res_ES
dc.relationMINECO/ICTI2013-2016/SAF2014-53209-Res_ES
dc.relationS2013/MT-3029/NANOAVANSENS-
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectEndoglines_ES
dc.subjectCD105es_ES
dc.subjectHuman serumes_ES
dc.subjectElectrochemical immunosensor,es_ES
dc.subjectSerumes_ES
dc.titleAmperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodeses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.electacta.2018.10.015-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.electacta.2018.10.015es_ES
dc.embargo.terms2019-12-01es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrid-
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.contributor.orcidMartínez-Periñán, E. [0000-0003-3122-3381]es_ES
dc.contributor.orcidSánchez-Tirado, E. [0000-0002-2386-4552]es_ES
dc.contributor.orcidGonzález-Cortés, A. [0000-0001-9054-9993]es_ES
dc.contributor.orcidBarderas, Rodrigo [0000-0003-3539-7469]es_ES
dc.contributor.orcidSánchez-Puelles, José María [0000-0002-4283-4885]es_ES
dc.contributor.orcidCampuzano, Susana [0000-0002-9928-6613]es_ES
dc.contributor.orcidPingarrón, J.M. [0000-0003-2271-1383]es_ES
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