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Spatial and temporal distribution of harmful microalgae detected with HTS metabarcoding

AuthorsTomasino, Maria Paola; Piredda, Roberta; Sánchez, Pablo ; Duarte, Bernardo; Fosso, Bruno; Caçador, Isabel; Amaral-Zettler, Linda; Sarno, Diana; Pesole, Graziano; Zingone, Adriana
Issue Date9-Oct-2016
CitationICHA : The 17th International Conference on Harmful Algae : Abstract Book: 64 (2016)
AbstractEnvironmental DNA analysis is a powerful means for assessing biodiversity, allowing the quasi-exhaustive detection of multiple species in a relatively rapid, accurate and simultaneous way. Here we tested the feasibility and effectiveness of High throughput sequencing (HTS)-DNA metabarcoding to assess Harmful Microalgae distribution across temporal and spatial scales. To this aim we first built up a custom Harmful Microalgae 18S reference database (18S-HMa db) including manually checked sequences available for 95 of the 113 known HMa species and for their non-toxic congeners. Then an 18S-V4 HMa sub-database was generated, which consisted of 642 reference sequences among which, V4 haplotypes shared among different species were labelled. The V4-18S HMa db was then used to analyse: i) The Ocean Sampling Day (OSD) 18S-V4 dataset, collected on 21 June 2014 at 157 world-wide distributed coastal sites. In this dataset we detected 49 different HMa species across 147 OSD samples, and identified the most frequent HMa species along with the hot-spot sites for HMa diversity. ii) The long term MareChiara station (LTER-MC, Gulf of Naples, Mediterranean Sea) 18S-V4 dataset, obtained from 48 samples collected over three years (2011-2013). In this dataset 26 HMa species were detected, three of which never recorded before in the area. For the most abundant species, seasonal patterns matched those recorded using light microscopy. These results suggest that, despite some limitations, HTS-DNA metabarcoding is an appropriate tool to be applied to the detection of harmful microalgae in monitoring programs and for the assessment of their geographic ranges
Description17th International Conference on Harmful Algae (ICHA), 9-14 October 2016, Florianópolis, Santa Catarina, Brazil.-- 1 page
Publisher version (URL)http://icha2016.com/program/#collapseOne
Appears in Collections:(ICM) Comunicaciones congresos
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