Description of Oxalicibacterium horti sp. nov. and Oxalicibacterium faecigallinarum sp. nov., new aerobic, yellow-pigmented, oxalotrophic bacteria

Abstract

Three strains of aerobic, Gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacteria (OD1T, YOxT and NS13), which were isolated in previous studies by enrichment in a mineral medium with potassium oxalate as the sole carbon source, were characterized. On the basis of 16S rRNA gene sequence similarity, strains OD1T, YOxT and NS13 belong to the Betaproteobacteria, most closely related to Oxalicibacterium flavum TA17T (97.2–99.7% sequence similarity). The major whole-cell fatty acids were C16:0, C16:1ω7c and C17:0 cyclo. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains OD1T and YOxT from O. flavum TA17T and from each other. Therefore, it is concluded that the strains OD1T and YOxT represent novel species within the genus Oxalicibacterium, for which the names Oxalicibacterium horti sp. nov. (type strain OD1T=DSM 21640T=NBRC 13594T) and Oxalicibacterium faecigallinarum sp. nov. (type strain YOxT=DSM 21641T=CCM 2767T) are proposed