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Título

Identification of the repressor subdomain within the signal reception module of the prokaryotic enhancer-binding protein XylR of Pseudomonas putida

AutorPérez-Martín, José CSIC ORCID ; Lorenzo, Víctor de CSIC ORCID
Palabras claveTol plasmid
Nucleotide-sequence
Promoters
Family
Fecha de publicación5-abr-1996
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónThe Journal of Biological Chemistry 271:7899-7902 (1996)
ResumenIn the presence of m-xylene, the protein XylR encoded by the TOL plasmid of Pseudomonas putida, activates the GraphicGraphic-dependent promoter Pu. Early activation stages involve the release of the intramolecular repression caused by the signal reception N-terminal (A domain) of XylR on the central module of the protein. A genetic approach has been followed to locate the specific segment within A domain of XylR that is directly responsible for its down-regulation in the absence of inducer, as compared to that involved in effector (m-xylene) binding. For this, a reporter Escherichia coli strain carrying a monocopy transcriptional fusion of Pu to lacZ was transformed with a collection of plasmids encoding equivalent truncated varieties of XylR, consisting of nested and internal deletions throughout the entire A domain. Examination of the resulting phenotypes allowed the assignment of the A domain region near the central activation domain, as the portion of the protein responsible for the specific repression of XylR activity in the absence of m-xylene.
Descripción5 p.-3 fig.
Versión del editorhttp://dx.doi.org/ 10.1074/jbc.271.14.7899
URIhttp://hdl.handle.net/10261/170153
DOI10.1074/jbc.271.14.7899
ISSN0021-9258
E-ISSN1083-351X
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