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Título: | Identification of the repressor subdomain within the signal reception module of the prokaryotic enhancer-binding protein XylR of Pseudomonas putida |
Autor: | Pérez-Martín, José CSIC ORCID ; Lorenzo, Víctor de CSIC ORCID | Palabras clave: | Tol plasmid Nucleotide-sequence Promoters Family |
Fecha de publicación: | 5-abr-1996 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | The Journal of Biological Chemistry 271:7899-7902 (1996) | Resumen: | In the presence of m-xylene, the protein XylR encoded by the TOL plasmid of Pseudomonas putida, activates the GraphicGraphic-dependent promoter Pu. Early activation stages involve the release of the intramolecular repression caused by the signal reception N-terminal (A domain) of XylR on the central module of the protein. A genetic approach has been followed to locate the specific segment within A domain of XylR that is directly responsible for its down-regulation in the absence of inducer, as compared to that involved in effector (m-xylene) binding. For this, a reporter Escherichia coli strain carrying a monocopy transcriptional fusion of Pu to lacZ was transformed with a collection of plasmids encoding equivalent truncated varieties of XylR, consisting of nested and internal deletions throughout the entire A domain. Examination of the resulting phenotypes allowed the assignment of the A domain region near the central activation domain, as the portion of the protein responsible for the specific repression of XylR activity in the absence of m-xylene. | Descripción: | 5 p.-3 fig. | Versión del editor: | http://dx.doi.org/ 10.1074/jbc.271.14.7899 | URI: | http://hdl.handle.net/10261/170153 | DOI: | 10.1074/jbc.271.14.7899 | ISSN: | 0021-9258 | E-ISSN: | 1083-351X |
Aparece en las colecciones: | (CIB) Artículos |
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J. Biol. Chem.-1996-Pérez-Martín-7899-902.pdf | Artículo principal | 71,79 kB | Adobe PDF | Visualizar/Abrir |
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