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Título: | Characterizing the Transcriptional Effects of Endolysin Treatment on Established Biofilms of Staphylococcus aureus |
Autor: | Fernández Llamas, Lucía CSIC ORCID; González Menéndez, Silvia CSIC ORCID; Gutiérrez, Diana CSIC ORCID; Campelo, Ana B. CSIC; Martínez Fernández, Beatriz CSIC ORCID ; Rodríguez González, Ana CSIC ORCID; García Suárez, María Pilar CSIC ORCID | Palabras clave: | Biofilms Endolysins Staphylococcus aureus RNA-seq Responses to antimicrobials |
Fecha de publicación: | 20-jun-2018 | Editor: | Bio-protocol | Citación: | Bio-Protocol 8(12): e2891 (2018) | Resumen: | Biofilms are the most common lifestyle of bacteria in both natural and human
environments. The organized structure of these multicellular communities generally protects bacterial
cells from external challenges, thereby enhancing their ability to survive treatment with antibiotics or
disinfectants. For this reason, the search for new antibiofilm strategies is an active field of study. In
this context, bacteriophages (viruses that infect bacteria) and their derived proteins have been
proposed as promising alternatives for eliminating biofilms. For instance, endolysins can degrade
peptidoglycan and, ultimately, lyse the target bacterial cells. However, it is important to characterize
the responses of bacterial cells exposed to these compounds in order to improve the design of
phage-based antimicrobial strategies. This protocol was developed to examine the transcriptional responses of Staphylococcus aureus biofilm cells exposed to endolysin treatment, as previously described in Fernández et al. (2017). However, it may be subsequently adapted to analyze the response of other microorganisms to different antimicrobials. |
Versión del editor: | https://doi.org/10.21769/BioProtoc.2891 | URI: | http://hdl.handle.net/10261/170038 | DOI: | 10.21769/BioProtoc.2891 | E-ISSN: | 2331-8325 |
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