English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/169770
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures

AuthorsMëlida, Hugo; Largo-Gosens, Asier; Novo-Uzal, Esther; Santiago Carabelos, Rogelio ; Pomar, F.; García, Pedro ; García-Angulo, Penélope; Acebes, José Luis; Alvarez, Jesús; Encina, Antonio
KeywordsCellulose
Dichlobenil
Ectopic lignin
Maize
Issue Date4-Mar-2015
PublisherWiley-Blackwell
CitationJournal of Integrative Plant Biology 57 (4): 357–372 (2015)
AbstractMaize (Zea mays L.) suspension‐cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor. Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy. Cell wall compositional analysis indicated that the cellulose‐deficient cell walls showed an enhancement of highly branched and cross‐linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p‐coumaric acid, and the presence of a polymer that stained positive for phloroglucinol. In accordance with this, cellulose‐deficient cell walls showed a fivefold increase in Klason‐type lignin. Thioacidolysis/GC‐MS analysis of cellulose‐deficient cell walls indicated the presence of a lignin‐like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress‐related lignin that arose in response to short‐term DCB‐treatments. Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin. A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions. In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
Description18 páginas, 8 figuras y 5 tablas.
Publisher version (URL)http://dx.doi.org/10.1111/jipb.12346
URIhttp://hdl.handle.net/10261/169770
DOI10.1111/jipb.12346
ISSN1672-9072
E-ISSN1744-7909
Appears in Collections:(MBG) Artículos
Files in This Item:
File Description SizeFormat 
Melida_Ectopic_lignification..pdfArtículo principal2,57 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.