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dc.contributor.authorMata, J. A.es_ES
dc.contributor.authorMoyano, Francisco Javieres_ES
dc.contributor.authorMartínez-Rodríguez, Gonzaloes_ES
dc.contributor.authorYúfera, Manueles_ES
dc.identifier.citationComparative Biochemistry and Physiology - A - Molecular and Integrative Physiology 197: 43-51 (2016)es_ES
dc.description.abstractIn order to identify daily changes in digestive physiology in developing gilthead seabream larvae, the enzyme activity (trypsin, lipases and α-amylase) and gene expression (trypsinogen-try, chymotrypsinogen-ctrb, bile salt-activated lipase-cel1b, phospholipase A2-pla2 and α-amylase-amy2a) were measured during a 24 h cycle in larvae reared under a 12 h light/12 h dark photoperiod. Larvae were sampled at 10, 18, 30 and 60 days post-hatch. In each sampling day, larvae were sampled every 3 h during a complete 24 h cycle. The enzyme activity and gene expression exhibited a marked dependent behavior to the light/darkness cycle in all tested ages. The patterns of activity and expression of all tested enzymes were compared to the feeding pattern found in the same larvae, which showed a rhythmic feeding pattern with a strong light synchronization. In the four tested ages, the activities of trypsin, and to a lesser extent lipases and amylase, were related to feeding activity. Molecular expression of the pancreatic enzymes tended to increase during the night, probably as an anticipation of the forthcoming ingestion of food that will take place during the next light period. It follows that the enzymatic activities are being regulated at translational and/or post-translational level. The potential variability of enzyme secretion along the whole day is an important factor to take into account in future studies. A particularly striking consequence of the present results is the reliability of studies based in only one daily sample taken at the same hour of the day, as those focused to assess ontogeny of digestive enzymes.es_ES
dc.description.sponsorshipThis research was funded by the Spanish Ministry of Economic Affairs and Competitiveness (MINECO) by project RIDIGEST (AGL2011-23722) with FEDER/ERDF contribution. J.A. Mata-Sotres was supported by a doctoral grant (ID 215473) from the Mexican National Council for Science and Technology (CONACYT).es_ES
dc.subjectDigestive enzymeses_ES
dc.subjectDaily rhythmses_ES
dc.subjectFish larvaees_ES
dc.subjectDigestive physiologyes_ES
dc.subjectSparus aurataes_ES
dc.titleDaily rhythms of digestive enzyme activity and gene expression in gilthead seabream (Sparus aurata) during ontogenyes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderConsejo Nacional de Ciencia y Tecnología (México)es_ES
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