Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/169616
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Moyano, José V. | es_ES |
dc.contributor.author | Carnemolla, Barbara | es_ES |
dc.contributor.author | Albar, Juan Pablo | es_ES |
dc.contributor.author | Leprini, Alessandra | es_ES |
dc.contributor.author | Gaggero, Barbara | es_ES |
dc.contributor.author | Zardi, Luciano | es_ES |
dc.contributor.author | García-Pardo, Angeles | es_ES |
dc.date.accessioned | 2018-09-12T10:43:22Z | - |
dc.date.available | 2018-09-12T10:43:22Z | - |
dc.date.issued | 1999-01-01 | - |
dc.identifier.citation | The Journal of Biological Chemistry 274 (1)135-142 (1999) | es_ES |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/169616 | - |
dc.description | 9 p.-9 fig.-1 tab. | es_ES |
dc.description.abstract | We recently reported that the heparin (Hep) III domain of fibronectin contains the H2 cell adhesion site in repeat III5 which binds activated α4 integrins. We have now further characterized the heparin and cell binding activities of this domain. A recombinant fragment containing repeats III4-III5 (FN-III4–5) induced Jurkat cell adhesion upon integrin activation with Mn2+ or TS2/16 monoclonal antibody (anti-β1). Adhesion of Mn2+-treated cells to FN-III4–5 or FN-III5 fragments was inhibited by chondroitinase ABC and ACII but not by the anti-α4 monoclonal antibody HP2/1. In contrast, HP2/1 completely blocked adhesion of TS2/16-treated cells while chondroitinase had a partial (FN-III4–5) or minor (FN-III5) effect. Thus, the role of each receptor depended on the stimulus used to activate α4β1. The combination of HP2/1 and chondroitinase at dilutions which did not inhibit when used individually abolished adhesion of Mn2+ or TS2/16-treated cells to both fragments, indicating a cooperative effect between α4β1 and chondroitin sulfate proteoglycans (CSPG). Furthermore, we have identified a 20-amino acid sequence in III5 (HBP/III5) which binds heparin and induces cell adhesion via CSPG exclusively. Although soluble HBP/III5 was a poor inhibitor, when combined with H2, it abolished adhesion to FN-III4–5 and FN-III5 fragments. These results establish that adhesion to the Hep III domain involves the cooperation of activated α4β1 and CSPG and show that HBP/III5 is a novel heparin and CSPG-binding site contributing to cell adhesion to this domain. | es_ES |
dc.description.sponsorship | This work was supported by Grants SAF97-0064-C03-02 from the Comisión Interministerial de Ciencia y Tecnología (CICYT), 94/0277 from Fondo de Investigaciones Sanitarias (FIS), and partially by funds from the Associazione Italiana per la Ricerca sul Cancro (to L. Z). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Human-plasma fibronectin | es_ES |
dc.subject | fn-c/h-v | es_ES |
dc.subject | Synthetic peptide | es_ES |
dc.subject | Monoclonal-antibody | es_ES |
dc.subject | Melanoma adhesion | es_ES |
dc.subject | Region | es_ES |
dc.subject | Recognition | es_ES |
dc.subject | Fibroblasts | es_ES |
dc.subject | Receptor | es_ES |
dc.subject | Progenitors | es_ES |
dc.title | Cooperative role for activated alpha 4 beta 1 integrin and chondroitin sulfate proteoglycans in cell adhesion to the heparin III domain of fibronectin - Identification of a novel heparin and cell binding sequence in repeat III5 | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1074/jbc.274.1.135 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.274.1.135 | es_ES |
dc.identifier.e-issn | 1083-351X | - |
dc.contributor.funder | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Associazione Italiana per la Ricerca sul Cancro | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100005010 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100007273 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.contributor.orcid | Moyano, José V. [0000-0003-1009-8059] | es_ES |
dc.contributor.orcid | Zardi, Luciano [0000-0002-7025-5303] | es_ES |
dc.contributor.orcid | García-Pardo, Angeles [0000-0001-5577-2954] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
J. Biol. Chem.-1999-Moyano-135-42.pdf | Artículo principal | 333,97 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
40
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
38
checked on 23-feb-2024
Page view(s)
226
checked on 18-abr-2024
Download(s)
153
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.