English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/169294
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood

AuthorsBlanco, Elena; Pérez-Andrés, Martin; Arriba-Méndez, Sonia; Contreras-Sanfeliciano, Teresa; Criado, Ignacio; Pelak, Ondrej; Serra-Caetano, Ana; Romero, Alfonso; Puig, Noemi; Remesal, Ana; Torres Canizales, Juan; López-Granados, Eduardo; Kalina, Tomas; Sousa, Ana E.; Zelm, Menno C. van; Burg, Mirjam van der; Dongen, J. J. M. van; Orfao, Alberto
Issue Date2018
PublisherElsevier
CitationJournal of Allergy and Clinical Immunology 141(6): 2208-2219.e16 (2018)
Abstract[Background]: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory B-cell (MBC) and plasma cell (PCs) compartments remains to be investigated. [Objective]: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. [Methods]: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n = 19) and peripheral blood (n = 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. [Results]: IgH-switched MBCs expressing IgG, IgG, IgG, IgA, and IgA were already detected in cord blood and newborns at very low counts, whereas CD27IgMIgD MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG, IgG, and IgA) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG, IgG, and IgA) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG, IgG, IgG, IgA, and IgA; until 2 to 4 years for IgD; and until 5 to 9 years for IgG and decreasing thereafter. For most IgH isotypes (except IgD and IgG), maximum plasma levels were reached after PC and MBC counts peaked. [Conclusions]: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life.
DescriptionOn behalf of the EuroFlow PID group.
Publisher version (URL)https://doi.org/10.1016/j.jaci.2018.02.017
URIhttp://hdl.handle.net/10261/169294
Identifiersdoi: 10.1016/j.jaci.2018.02.017
e-issn: 1097-6825
issn: 0091-6749
Appears in Collections:(IBMCC) Artículos
Files in This Item:
File Description SizeFormat 
ageblood.pdf4,78 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.