English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/169229
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A systematic approach for peptide characterization of B-cell receptor in chronic lymphocytic leukemia cells

AuthorsDíez, Paula; Ibarrola, Nieves ; Dégano, Rosa M.; Lécrevisse, Quentin; Rodríguez-Caballero, Arancha; Criado, Ignacio; Nieto, Wendy G.; Góngora, Rafael; González, Marcos ; Almeida, Julia ; Orfao, Alberto ; Fuentes, Manuel
KeywordsPeptide sequencing
Mass spectrometry
Immunoglobulin
B-cell receptor
Chronic lymphocytic leukemia
Issue Date2017
PublisherImpact Journals
CitationOncotarget 8(26): 42836-42846 (2017)
AbstractA wide variety of immunoglobulins (Ig) is produced by the immune system thanks to different mechanisms (V(D)J recombination, somatic hypermutation, and antigen selection). The profiling of Ig sequences (at both DNA and peptide levels) are of great relevance to developing targeted vaccines or treatments for specific diseases or infections. Thus, genomics and proteomics techniques (such as Next- Generation Sequencing (NGS) and mass spectrometry (MS)) have notably increased the knowledge in Ig sequencing and serum Ig peptide profiling in a high-throughput manner. However, the peptide characterization of membrane-bound Ig (e.g., B-cell receptors, BCR) is still a challenge mainly due to the poor recovery of mentioned Ig. Herein, we have evaluated three different sample processing methods for peptide sequencing of BCR belonging to chronic lymphocytic leukemia (CLL) B cells identifying up to 426 different peptide sequences (MS/MS data are available via ProteomeXchange with identifier PXD004466). Moreover, as a consequence of the results here obtained, recommended guidelines have been described for BCR-sequencing of B-CLL samples by MS approaches. For this purpose, an in-house algorithm has been designed and developed to compare the MS/MS results with those obtained by molecular biology in order to integrate both proteomics and genomics results and establish the steps to follow when sequencing membrane-bound Ig by MS/MS.
Publisher version (URL)https://doi.org/10.18632/oncotarget.17076
URIhttp://hdl.handle.net/10261/169229
Identifiersdoi: 10.18632/oncotarget.17076
e-issn: 1949-2553
Appears in Collections:(IBMCC) Artículos
Files in This Item:
File Description SizeFormat 
sytemacell.pdf2,73 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.