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Naturally occurring quaternary benzo[c]phenanthridine alkaloids selectively stabilize G-quadruplexes

AuthorsJarosova, Petra; Paroulek, Petr; Rajecky, Michal; Rajecka, Veronika; Taborska, Eva; Aviñó, Anna ; Eritja Casadellà, Ramón ; Mazzini, Stefania; Gargallo, Raimundo; Taborsky, Petr
DNA structures
Issue Date6-Aug-2018
PublisherRoyal Society of Chemistry (UK)
CitationPhysical Chemistry Chemical Physics 20: 21772-21782 (2018)
AbstractIn this work, the interaction of six natural benzo[c]phenanthridine alkaloids (macarpine, sanguilutine, sanguirubine, chelerythrine, sanguinarine and chelirubine) with parallel and antiparallel G-quadruplex DNA structures was studied. HT22 corresponding to the end of human telomeres and the modified promoter oncogene c-kit21 and Pu22 sequences have been used. Spectroscopically-monitored melting experiments and fluorescence titrations, competitive dialysis and nuclear magnetic resonance spectroscopy were used for this purpose. The results showed that these alkaloids stabilized G-quadruplex structures in terms of increments of Tm values (from 15 to 25 °C) with high selectivity over duplexes and unfolded DNA. The mode of binding was mainly by stacking on the terminal G-tetrads with stoichiometries of 1 : 2 (DNA : ligand). The presence of non-specific electrostatic interactions was also observed. Overall, the results pointed to a strong stabilization of G-quadruplex structures by these alkaloids.
Publisher version (URL)10.1039/C8CP02681E
Appears in Collections:(IQAC) Artículos
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