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dc.contributor.authorRamos, Teresa L.-
dc.contributor.authorSánchez-Abarca, Luis Ignacio-
dc.contributor.authorRosón, Beatriz-
dc.contributor.authorRedondo, Alba-
dc.contributor.authorRico, Ana-
dc.contributor.authorPreciado, Silvia-
dc.contributor.authorOrtega, Rebeca-
dc.contributor.authorRodríguez, Concepción-
dc.contributor.authorMuntión, Sandra-
dc.contributor.authorHernández-Hernández, Angel-
dc.contributor.authorDe Las Rivas, Javier-
dc.contributor.authorGonzález, Marcos-
dc.contributor.authorGonzález-Porras, José R.-
dc.contributor.authorCañizo, María Consuelo del-
dc.contributor.authorSánchez-Guijo, Fermín M.-
dc.identifierdoi: 10.1371/journal.pone.0182470-
dc.identifiere-issn: 1932-6203-
dc.identifier.citationPLoS ONE 12(8): e0182470 (2017)-
dc.description.abstractThere is evidence of continuous bidirectional cross-talk between malignant cells and bone marrow-derived mesenchymal stromal cells (BM-MSC), which favors the emergence and progression of myeloproliferative neoplastic (MPN) diseases. In the current work we have compared the function and gene expression profile of BM-MSC from healthy donors (HD-MSC) and patients with MPN (JAK2V617F), showing no differences in the morphology, proliferation and differentiation capacity between both groups. However, BM-MSC from MPN expressed higher mean fluorescence intensity (MIF) of CD73, CD44 and CD90, whereas CD105 was lower when compared to controls. Gene expression profile of BM-MSC showed a total of 169 genes that were differentially expressed in BM-MSC from MPN patients compared to HD-MSC. In addition, we studied the ability of BM-MSC to support the growth and survival of hematopoietic stem/progenitor cells (HSPC), showing a significant increase in the number of CFU-GM colonies when MPN-HSPC were co-cultured with MPN-MSC. Furthermore, MPN-MSC showed alteration in the expression of genes associated to the maintenance of hematopoiesis, with an overexpression of SPP1 and NF-kB, and a downregulation of ANGPT1 and THPO. Our results suggest that BM-MSC from JAK2 patients differ from their normal counterparts and favor the maintenance of malignant clonal hematopoietic cells.-
dc.description.sponsorshiperesa Lopes Ramos is supported by a fellowship by the Portuguese Fundação para a Ciência e Tecnologia (SFRH/BD/86451/2012). Sandra Muntion is supported by Red TerCel from Instituto de Salud Carlos III (RD12/0019/0017). Silvia Preciado is supported by a Universidad de Salamanca-Banco de Santander joint grant. This study was partially supported by grant GRS 1034/A/14 and BIO/SA28/14 from Consejería de Sanidad de la Junta de Castilla y León.-
dc.publisherPublic Library of Science-
dc.relation.isversionofPublisher's version-
dc.titleMesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis-
dc.description.versionPeer Reviewed-
dc.contributor.funderJunta de Castilla y León-
dc.contributor.funderUniversidad de Salamanca-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderFundação para a Ciência e a Tecnologia (Portugal)-
dc.contributor.funderBanco Santander-
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