English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/169064
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis

AuthorsInfantes-Lorenzo, José Antonio; Moreno, Inmaculada; Risalde, María Ángeles; Roy, Álvaro; Villar, Margarita ; Romero, Beatriz; Ibarrola, Nieves ; Fuente, José de la ; Puentes, Eugenia; Juan, Lucía de; Gortázar, Christian ; Bezos, Javier; Domínguez, Lucas; Domínguez, Mercedes
KeywordsProteome
Cross-reaction
P22
PPD
Tuberculosis
Issue Date2017
PublisherBioMed Central
CitationClinical Proteomics 14: 36 (2017)
Abstract[Background]: Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between Mycobacterium bovis (M. bovis) and M. avium proteins. Thus, the objective of this study was to carry out a proteomic characterisation of bPPD, aPPD and an immunopurified subcomplex from bPPD called P22 in order to identify proteins contributing to cross-reactivity among these three products in tuberculosis diagnosis. [Methods]: Trypsin digests of bPPD, aPPD and P22 were analysed by nanoscale liquid chromatography-electrospray ionization tandem mass spectrometry. Mice were immunised with bPPD or aPPD, and their serum was tested by indirect ELISA for reactivity against these preparations as well as against P22. [Results]: A total of 456 proteins were identified in bPPD, 1019 in aPPD and 118 in P22; 146 of these proteins were shared by bPPD and aPPD, and 43 were present in all three preparations. Candidate proteins that may cause cross-reactivity between bPPD and aPPD were identified based on protein abundance and antigenic propensity. Serum reactivity experiments indicated that P22 may provide greater specificity than bPPD with similar sensitivity for ELISA-type detection of antibodies against M. tuberculosis complex. [Conclusion]: The subpreparation from bPPD called P22 may be an alternative to bPPD for serodiagnosis of bovine tuberculosis, since it shares fewer proteins with aPPD than bPPD does, reducing risk of cross-reactivity with anti-M. avium antibodies.
Publisher version (URL)https://doi.org/10.1186/s12014-017-9171-z
URIhttp://hdl.handle.net/10261/169064
Identifiersdoi: 10.1186/s12014-017-9171-z
e-issn: 1559-0275
issn: 1542-6416
Appears in Collections:(IBMCC) Artículos
(IREC) Artículos
Files in This Item:
File Description SizeFormat 
protetuber.pdf1,07 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.