English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/168694
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

RasGRF2 controls nuclear migration in postnatal retinal cone photoreceptors

AuthorsJimeno, David ; Gómez, Carmela; Calzada, Nuria ; Villa, Pedro de la; Lillo, Concepción; Santos de Dios, Eugenio
KeywordsNuclear migration
Cone photoreceptor
GRF2
Ras signaling
GEF
GRF1
Intracellular traffic
Retina
Issue Date2016
PublisherCompany of Biologists
CitationJournal of Cell Science 129(4): 729-742 (2016)
AbstractDetailed immunocytochemical analyses comparing wild-type (WT), GRF1-knockout (KO), GRF2-KO and GRF1/2 double-knockout (DKO) mouse retinas uncovered the specific accumulation of misplaced, 'ectopic' cone photoreceptor nuclei in the photoreceptor segment (PS) area of retinas from GRF2-KO and GRF1/2-DKO, but not of WT or GRF1-KO mice. Localization of ectopic nuclei in the PS area of GRF2-depleted retinas occurred postnatally and peaked between postnatal day (P)11 and P15. Mechanistically, the generation of this phenotype involved disruption of the outer limiting membrane and intrusion into the PS layer by cone nuclei displaying significant perinuclear accumulation of signaling molecules known to participate in nuclear migration and cytoskeletal reorganization, such as PAR3, PAR6 and activated, phosphorylated forms of PAK, MLC2 and VASP. Electroretinographic recordings showed specific impairment of cone-mediated retinal function in GRF2-KO and GRF1/2-DKO retinas compared with WT controls. These data identify defective cone nuclear migration as a novel phenotype in mouse retinas lacking GRF2 and support a crucial role of GRF2 in control of the nuclear migration processes required for proper postnatal development and function of retinal cone photoreceptors.
Publisher version (URL)https://doi.org/10.1242/jcs.180919
URIhttp://hdl.handle.net/10261/168694
Identifiersdoi: 10.1242/jcs.180919
issn: 0021-9533
e-issn: 1477-9137
Appears in Collections:(IBMCC) Artículos
Files in This Item:
File Description SizeFormat 
rasgrf2.pdf10,28 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.