English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/168498
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Título

The circadian transcriptome of marine fish (Sparus aurata) larvae reveals highly synchronized biological processes at the whole organism level

AutorYúfera, Manuel ; Perera, Erick; Mata, J. A. ; Calduch-Giner, Josep A. ; Martínez-Rodríguez, Gonzalo ; Pérez-Sánchez, Jaume
Fecha de publicación11-oct-2017
EditorSpringer Nature
CitaciónScientific Reports 7: 12943 (2017)
ResumenThe regulation of circadian gene expression remains largely unknown in farmed fish larvae. In this study, a high-density oligonucleotide microarray was used to examine the daily expression of 13,939 unique genes in whole gilthead sea bream (Sparus aurata) larvae with fast growth potentiality. Up to 2,229 genes were differentially expressed, and the first two components of Principal Component Analysis explained more than 81% of the total variance. Clustering analysis of differentially expressed genes identified 4 major clusters that were triggered sequentially, with a maximum expression at 0 h, 3 h, 9–15 h and 18-21 h zeitgeber time. Various core clock genes (per1, per2, per3, bmal1, cry1, cry2, clock) were identified in clusters 1–3, and their expression was significantly correlated with several genes in each cluster. Functional analysis revealed a daily consecutive activation of canonical pathways related to phototransduction, intermediary metabolism, development, chromatin remodeling, and cell cycle regulation. This daily transcriptome of whole larvae resembles a cell cycle (G1/S, G2/M, and M/G1 transitions) in synchronization with multicellular processes, such as neuromuscular development. This study supports that the actively feeding fish larval transcriptome is temporally organized in a 24-h cycle, likely for maximizing growth and development.
Versión del editorhttps://doi.org/10.1038/s41598-017-13514-w
URIhttp://hdl.handle.net/10261/168498
DOI10.1038/s41598-017-13514-w
E-ISSN2045-2322
Aparece en las colecciones: (ICMAN) Artículos
(IATS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
sparus_aurata_Yufera.pdf2,79 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.