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Transforming growth factor-α stimulates insulin-like growth factor binding protein-4 (IGFBP-4) expression and blocks follicle-stimulating hormone regulation of IGFBP-4 production in rat granulosa cells

AuthorsPiferrer, Francesc ; Li, D.; Shimasaki, S.; Erickson, G.F.
IGFBP-4 Protease
Granulosa cells
Protein expression
Hormone inhibition
Transforming growth factor alpha
Somatomedin binding protein 4
Issue Date30-Sep-1997
CitationMolecular and Cellular Endocrinology 133(1): 9-17 (1997)
AbstractThe ability of TGF-α to regulate insulin-like growth factor binding protein-4 (IGFBP-4), was investigated. Primary cultures of rat granulosa cells (GC) were grown in serum-free medium with rat (r) TGF-α and/or rFSH, and secreted IGFBP-4 protein and its steady state mRNA levels were measured by Western immunoblotting and Northern blotting, respectively. Control (untreated) cells secreted IGFBP-4 spontaneously, and the levels were increased by rTGF-α in a dose- and time-dependent manner. rTGF-α abolished FSH-induced IGFBP-4 protease activity and suppressed FSH-dependent effects on IGFBP-4 production. IGFBP-4 mRNA levels were decreased and increased by FSH and TGF-α, respectively, and TGF-α blocked the FSH effects. These results demonstrate that TGF-α is a potent stimulator of IGFBP-4 expression in rat GC and can overcome the regulatory effects of FSH on IGFBP-4 production. The consequence of these TGF-α effects is a marked, sustained increase in the levels of IGFBP-4 in the microenvironment.
Description9 pages, 7 figures.-- PMID: 9359468 [PubMed].
Publisher version (URL)http://dx.doi.org/10.1016/S0303-7207(97)00139-1
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