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Closed Access item A microsatellite genetic map of the turbot (Scophthalmus maximus)

Authors:Bouza, Carmen
Hermida, Miguel
Pardo, Belén G.
Fernández, Carlos
Fortes, Gloria G.
Castro, Jaime
Sánchez, Laura
Presa, Pablo
Pérez, Montse
Sanjuán, Andrés
Carlos, Alejandro de
Álvarez-Dios, José Antonio
Ezcurra, Susana
Cal, Rosa M.
Piferrer, Francesc
Martínez, Paulino
Issue Date:Dec-2007
Publisher:Genetics Society of America
Citation:Genetics 177: 2457-2467 (2007)
Abstract:A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an average distance between markers of 6.5 ± 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revealed significant differences between sexes, ~1.6 times higher in the female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81–100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will be suitable for QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.
Description:11 pages, 3 figures, 2 tables.-- PMID: 18073440 [PubMed].-- PMCID: PMC2219478.-- Supporting information available at: http://www.genetics.org/cgi/content/full/177/4/2457/DC1
Full-text version available Open Access via PubMed Central at: http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=2219478&blobtype=pdf
Publisher version (URL):http://dx.doi.org/10.1534/genetics.107.075416
URI:http://hdl.handle.net/10261/16822
ISSN:0016-6731 (Print)
1943-2361 (Online)
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Appears in Collections:(ICM) Artículos

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