English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/166588
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage

AuthorsSaugar, Irene ; Jiménez-Martín, Alberto; Tercero, José Antonio
Issue Date2017
CitationCell Reports 20: 1553- 1562 (2017)
AbstractStructure-specific endonucleases contribute to the maintenance of genome integrity by cleaving DNA intermediates that need to be resolved for faithful DNA repair, replication, or recombination. Despite advances in the understanding of their function and regulation, it is less clear how these proteins respond to genotoxic stress. Here, we show that the structurespecific endonuclease Mus81-Mms4/EME1 relocalizes to subnuclear foci following DNA damage and colocalizes with the endonucleases Rad1-Rad10 (XPF-ERCC1) and Slx1-Slx4. Recruitment takes place into a class of stress foci defined by Cmr1/WDR76, a protein involved in preserving genome stability, and depends on the E2-ubiquitin-conjugating enzyme Rad6 and the E3-ubiquitin ligase Bre1. Foci dynamics show that, in the presence of DNA intermediates that need resolution by Mus81-Mms4, Mus81 foci persist until this endonuclease is activated by Mms4 phosphorylation. Our data suggest that subnuclear relocalization is relevant for the function of Mus81-Mms4 and, probably, of the endonucleases that colocalize with it
URIhttp://hdl.handle.net/10261/166588
Identifiersdoi: 10.1016/j.celrep.2017.07.059
issn: 2211-1247
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
TerceroJA_SubneclarRelocalization.pdf3,51 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.