Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/166470
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Retrotransposons are specified as DNA replication origins in the gene-poor regions of Arabidopsis heterochromatin |
Autor: | Vergara, Zaida CSIC ORCID; Sequeira-Mendes, Joana CSIC ORCID; Morata, Jordi CSIC ORCID; Peiró, Ramón; Hénaff, Elizabeth CSIC; Costas, Celina; Casacuberta, Josep M. CSIC ORCID; Gutiérrez Armenta, Crisanto CSIC ORCID | Fecha de publicación: | 5-jun-2017 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 45: 8358- 8368 (2017) | Resumen: | Genomic stability depends on faithful genome replication. This is achieved by the concerted activity of thousands of DNA replication origins (ORIs) scattered throughout the genome. The DNA and chromatin features determining ORI specification are not presently known. We have generated a high-resolution genome-wide map of 3230 ORIs in cultured Arabidopsis thaliana cells. Here, we focused on defining the features associated with ORIs in heterochromatin. In pericentromeric gene-poor domains ORIs associate almost exclusively with the retrotransposon class of transposable elements (TEs), in particular of the Gypsy family. ORI activity in retrotransposons occurs independently of TE expression and while maintaining high levels of H3K9me2 and H3K27me1, typical marks of repressed heterochromatin. ORI-TEs largely colocalize with chromatin signatures defining GC-rich heterochromatin. Importantly, TEs with active ORIs contain a local GC content higher than the TEs lacking them. Our results lead us to conclude that ORI colocalization with retrotransposons is determined by their transposition mechanism based on transcription, and a specific chromatin landscape. Our detailed analysis of ORIs responsible for heterochromatin replication has implications on the mechanisms of ORI specification in other multicellular organisms in which retrotransposons are major components of heterochromatin and of the entire genome. | URI: | http://hdl.handle.net/10261/166470 | DOI: | 10.1093/nar/gkx524 | Identificadores: | doi: 10.1093/nar/gkx524 issn: 1362-4962 |
Aparece en las colecciones: | (CBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
GutierrezC_RetrotransposonsAreSpecified.pdf | 1,46 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
11
checked on 12-abr-2024
SCOPUSTM
Citations
14
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
14
checked on 29-feb-2024
Page view(s)
266
checked on 23-abr-2024
Download(s)
153
checked on 23-abr-2024