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Title

Detection of DNA-RNA hybrids in vivo

AuthorsGarcía-Rubio, María L. ; Barroso, Sonia ; Aguilera, Andrés
KeywordsDNA-RNA hybrids
Genome instability
R loop
S9.6 antibody
Issue Date2018
PublisherSpringer Nature
CitationGenome Instability: 347-361 (2018)
SeriesMethods in Molecular Biology 1672
AbstractDNA-RNA hybrids form naturally during essential cellular functions such as transcription and replication. However, they may be an important source of genome instability, a hallmark of cancer and genetic diseases. Detection of DNA-RNA hybrids in cells is becoming crucial to understand an increasing number of molecular biology processes in genome dynamics and function and to identify new factors and mechanisms responsible for disease in biomedical research. Here, we describe two different procedures for the reliable detection of DNA-RNA hybrids in the yeast Saccharomyces cerevisiae and in human cells: DNA-RNA Immunoprecipitation (DRIP) and Immunofluorescence.
URIhttp://hdl.handle.net/10261/166128
DOI10.1007/978-1-4939-7306-4_24
ISBN978-1-4939-7306-4
Appears in Collections:(CABIMER) Libros y partes de libros
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