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Título: | Structure of promoter-bound TFIID and model of human pre-initiation complex assembly |
Autor: | Louder, R.K.; He, Y.; López-Blanco, José R. CSIC ORCID; Fang, J.; Chacón, Pablo CSIC ORCID; Nogales, Eva | Fecha de publicación: | 2016 | Citación: | Nature 531: 604- 609 (2016) | Resumen: | The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1-13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP-TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation. | URI: | http://hdl.handle.net/10261/166074 | DOI: | 10.1038/nature17394 | Identificadores: | doi: 10.1038/nature17394 issn: 1476-4687 |
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