Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/165664
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Physical proximity of chromatin to nuclear pores prevents harmful R loop accumulation contributing to maintain genome stability

AutorGarcía-Benítez, Francisco CSIC; Gaillard, Hélène CSIC ORCID; Aguilera, Andrés CSIC ORCID
Palabras claveGenome instability
R loop
Transcription
Nuclear pores
Mpl1/2
Fecha de publicación2017
EditorNational Academy of Sciences (U.S.)
CitaciónProceedings of the National Academy of Sciences 114(41): 10942-10947 (2017)
ResumenDuring transcription, the mRNA may hybridize with DNA, forming an R loop, which can be physiological or pathological, constituting in this case a source of genomic instability. To understand the mechanism by which eukaryotic cells prevent harmful R loops, we used human activation-induced cytidine deaminase (AID) to identify genes preventing R loops. A screening of 400 Saccharomyces cerevisiae selected strains deleted in nuclear genes revealed that cells lacking the Mlp1/2 nuclear basket proteins show AID-dependent genomic instability and replication defects that were suppressed by RNase H1 overexpression. Importantly, DNA–RNA hybrids accumulated at transcribed genes in mlp1/2 mutants, indicating that Mlp1/2 prevents R loops. Consistent with the Mlp1/2 role in gene gating to nuclear pores, artificial tethering to the nuclear periphery of a transcribed locus suppressed R loops in mlp1Δ cells. The same occurred in THO-deficient hpr1Δ cells. We conclude that proximity of transcribed chromatin to the nuclear pore helps restrain pathological R loops.
URIhttp://hdl.handle.net/10261/165664
DOI10.1073/pnas.1707845114
Identificadoresdoi: 10.1073/pnas.1707845114
e-issn: 1091-6490
issn: 0027-8424
Aparece en las colecciones: (CABIMER) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

25
checked on 15-feb-2024

SCOPUSTM   
Citations

33
checked on 20-mar-2024

WEB OF SCIENCETM
Citations

31
checked on 26-feb-2024

Page view(s)

274
checked on 28-mar-2024

Download(s)

115
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.