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Plasmid conjugation-based logic gates

AuthorsCuevas, Ana ; González-Gutiérrez, Sheila; Rodríguez-Patón, Alfonso; Garcillán-Barcia, M. Pilar; Cruz, Fernando de la
Issue Date2017
CitationXL SEBBM Congress (2017)
AbstractConjugation is a natural communication mechanism among bacteria that relies in plasmids as vehicles. The conjugation hardware is highly specific and robust, allowing the independent transmission of different conjugative plasmids without interference. We constructed an E. coli derivative containing a chromosomally-inserted conjugative W-wire and engineered a recombinant plasmid series able to compute the basic logic gates YES, AND, OR. The computing strain was used as a donor in mating experiments and a reporter strain able to trigger the production of a fluorescent protein from the reporter plasmid was used as recipient. The functionality and specificity of these circuits constitute a proof of principle that cell-cell communication mediated by conjugative plasmids could be implemented as a new tool in Synthetic Biology to distribute genetic circuits in bacterial populations.
DescriptionResumen del póster presentado al XL Congreso de la Sociedad Española de Bioquímica y Biología Molecular (SEBBM), celebrado en Barcelona del 23 al 26 de octubre de 2017.
Appears in Collections:(IBBTEC) Comunicaciones congresos
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