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Analysis of the mechanism underlying the function of Sp6 and Sp8 in the limb ectoderm

AuthorsPérez-Gómez, Rocío; Fernández-Guerrero, Marc; Zunzunegui, Sandra ; López-Giménez, Juan F. ; Ros, María A.
Issue Date2016
PublisherSociedad Española de Biología Celular
Citation11th Meeting SEBD (2016)
AbstractWe have recently shown that the transcription factors Sp6 and Sp8 are conjointly necessary for limb development as no limbs form in their absence or substantial reduction. The study of the allelic series of double Sp6;Sp8 mutant also revealed that mice with only a functional copy of Sp8 exhibit the Split-hand/split-foot malformation. Based on the mo- lecular study of mutant limbs, we have postulated that Sp6 and Sp8 are necessary in the limb ectoderm, downstream of Wnt/ß-catenin, to activate Fgf8 and downstream of Bmp signaling, possibly cooperating with Smads for dorso-ven- tral patterning. Here we have used co-immunoprecipitation (CoIP) and bimolecular fluorescence complementation (BiFC) to explore these suspected protein-protein interactions. To this end, Sp6, Sp8 and Smads were tagged with Myc or Flag epitopes to their N-terminal end and with YFP full length or YFP moieties to their C-terminal end. By CoIP and BiFC we show that Sp6 and Sp8 can homo and heterodimerize and that they can also interact with Smads. Finally, by generating different truncated versions of Sp8 we resolve the protein regions responsible of these interaction. Our study underscores the mechanisms by which Sp6/8 meditare Wnt/ß -catenin and Bmp signaling in the limb ectoderm.
DescriptionResumen del póster presentado al 11th Meeting Spanish Society for Developmental Biology, celebrado en Girona del 19 al 21 de octubre de 2016.
Appears in Collections:(IBBTEC) Comunicaciones congresos
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