English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163892
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


C. elegans THSC complex deficiency leads to replication stress and genome instability

AuthorsGarcía-Muse, Tatiana ; Zheleva, Angelina; Galindo, Ulises; Aguilera, Andrés
Issue Date2017
CitationVI Spanish Worm Meeting (2017)
AbstractThe THSC/TREX-2 complex is conserved from yeast to humans and is involved in mRNP biogenesis. Defects in the coupling of transcription with mRNA processing and export results in DNA damage and genome instability from yeast to human cells. Notably, the genetic instability and transcriptional defects observed in yeast THSC mutants is due to R-loops accumulation as co-transcriptional cleavage of the transcript reduced to some extend both defects. However, recently our laboratory has show that depletion of human counterpart, despite increasing genomic instability, does not lead to an obvious accumulation of R-loops. Nevertheless, we decide to use the simple metazoan Caernorhabditis elegans to further address this unexpected data. Through the characterization of two putative members of the C. elegans THSC complex we have observed that lack of the THSC/TREX-2 THP-1 leads to, DNA damage accumulation and replication defects at the germline. Importantly, microinjection of RNase H, that removes R-loops, partially restores the Cy3-dUTP incorporation in the nematode germline consistent with R-loops interfering with replication progression. This research provides new insight into the THSC/TREX-2 complex contribution to the maintenance of genome integrity in multicellular organisms.
DescriptionResumen del trabajo presentado al VI Spanish Worm Meeting, celebrado en Valencia del 9 al 10 de marzo de 2017.
Appears in Collections:(CABIMER) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.