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Title

ATP synthesis coupled to the electroenzymatic activity of a hydrogenase immobilized at an electrode/biomimetic membrane interface

AuthorsLacey, Antonio L. de; Gutiérrez-Sanz, Óscar ; Natale, Paolo; Márquez, Ileana F. ; Marques, Marta C.; Zacarias, Sonia; Pita, Marcos ; Pereira, Inês A. C.; López-Montero, Iván; Vélez, Marisela
Issue DateJul-2017
CitationXXIV International Symposium on Bioelectrochemistry and Bioenergetics (2017)
AbstractCells generate energy by coupling a proton gradient across a phospholipid bilayer membrane with the activity of a cross-membrane ATP synthase enzyme. In an effort to mimic this process in an artificial environment, we show that ATP can be efficiently produced starting from molecular hydrogen as a fuel. The proton concentration in an electrode/phospholipid bilayer interface can be controlled and monitorised electrochemically by immobilizing the membrane-bound [NiFeSe]-hydrogenase from Desulfovibrio vulgaris Hildenborough.1 The electro-enzymatic oxidation of H2 generated a proton gradient across the supported biomimetic membrane that can be coupled to the in vitro synthesis of ATP by reconstituting ATP-synthase from E. coli on the biomimetic system.2
DescriptionTrabajo presentado en el XXIV International Symposium on Bioelectrochemistry and Bioenergetics (BES 2017), celebrado en Lyon del 3 al 7 de julio de 2017.
URIhttp://hdl.handle.net/10261/163874
Appears in Collections:(ICP) Comunicaciones congresos
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