English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163726
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Immobilization of Redox Enzymes on Nanoporous Gold Electrodes: Applications in Biofuel Cells

AuthorsSiepenkoetter, Till; Salaj‐Kosla, Urszula; Xiao, Xinxin; Ó Conghaile, Peter; Pita, Marcos ; Ludwig, Roland; Magner, Edmond
Issue DateApr-2017
PublisherWiley-VCH
CitationChemPlusChem 82(4): 553-560 (2017)
AbstractNanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold:silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD‐dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop‐casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium‐modified NPG electrode. The stability of the bilirubin‐oxidase‐modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx‐modified NPG cathode (500 nm thickness) and a GDH‐modified anode (300 nm), which generated power densities of 17.5 and 7.0 μW cm−2 in phosphate‐buffered saline and artificial serum, respectively.
Publisher version (URL)https://doi.org/10.1002/cplu.201600455
URIhttp://hdl.handle.net/10261/163726
DOI10.1002/cplu.201600455
E-ISSN2192-6506
Appears in Collections:(ICP) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.