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Title

Third-generation oxygen amperometric biosensor based on Trametes hirsuta laccase covalently bound to graphite electrode

AuthorsGutiérrez Sánchez, Cristina ; Shleev, Sergey; Lacey, Antonio L. de; Pita, Marcos
KeywordsOxygen biosensor
Direct electron transfer
Laccase
Bioelectrocatalysis
Issue DateJan-2015
PublisherWalter de Gruyter
CitationChemical Papers 69(1): 237-240 (2015)
AbstractThe response of low-density graphite electrodes hosting Trametes hirsuta laccase in a direct electron transfer regime is presented for real-time analysis of O2 concentrations. The use of contrasting immobilisation methods developed for biocathodes affords good reproducibility and reliability of the amperometric biosensor, which shows a limit of detection below 1 μM and a sensitivity slightly higher than 60 nA cm−2 M−1.
Publisher version (URL)https://doi.org/10.2478/s11696-014-0595-x
URIhttp://hdl.handle.net/10261/163700
DOI10.2478/s11696-014-0595-x
ISSN0366-6352
E-ISSN1336-9075
Appears in Collections:(ICP) Artículos
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