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dc.contributor.authorEstensoro, Itziares_ES
dc.contributor.authorPérez-Cordón, G.es_ES
dc.contributor.authorSitjà-Bobadilla, Ariadnaes_ES
dc.contributor.authorPiazzon de Haro, María Carlaes_ES
dc.date.accessioned2018-04-06T15:02:27Z-
dc.date.available2018-04-06T15:02:27Z-
dc.date.issued2018-04-
dc.identifier.citationJournal of Fish Diseaseses_ES
dc.identifier.urihttp://hdl.handle.net/10261/163279-
dc.description.abstractEnteromyxum leei is a myxozoan parasite responsible for enteritis in gilthead sea bream (Sparus aurata). The parasite proliferates in the paracellular space of the intestinal epithelium and induces an inflammatory reaction. In order to assess intestinal cell turnover and parasite proliferation, fish were infected with the parasite by anal intubation and after 17 and 64 days, the cell proliferative marker bromodeoxyuridine (BrdU) was administered and, after 24 hours, tissue samples were taken for immunohistochemical detection. Parasite exposure induced increased epithelial and immune cell proliferation in all intestinal segments at all time points, even before parasite establishment. This increased turnover was triggered early after intubation and mainly at a local level, as shown by an increased proliferating cell nuclear antigen (pcna) gene expression only at the posterior intestine after 17 days (not found in lymphohaematopietic organs). Incorporation of BrdU in parasite secondary and tertiary daughter cells indicated that parasite endogeny is not by schizogonial division, which uses de novo synthesis pathway of pyrimidines. Altogether, BrdU immunolabelling and pcna gene expression showed the rapid proliferative response of the fish intestines upon a myxozoan infection and how this response is effectively triggered even before the parasite reaches or establishes in the site.es_ES
dc.description.sponsorshipAGL2009-13282es_ES
dc.description.sponsorshipAGL2013-48560-Res_ES
dc.description.sponsorship634429es_ES
dc.description.sponsorshipPROMETEOII/2014/085es_ES
dc.description.sponsorshipAPOSTD/2016/037es_ES
dc.description.sponsorshipPIE project no.201740E013es_ES
dc.description.sponsorshipFPDI-2013-15741es_ES
dc.language.isoenges_ES
dc.relation634429es_ES
dc.relationAGL2013-48560-Res_ES
dc.relation.isversionofPostprintes_ES
dc.rightsclosedAccesses_ES
dc.subjectMyxozoaes_ES
dc.subjectImmunohistochemistryes_ES
dc.subjectIntestinees_ES
dc.subjectPCNAes_ES
dc.subjectSparus aurataes_ES
dc.titleBromodeoxyuridine DNA labelling reveals host and parasite proliferation in a fish-myxozoan modeles_ES
dc.typeartículoes_ES
dc.identifier.doi10.1111/jfd.12765-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversion10.1111/jfd.12765es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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