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Título

Bromodeoxyuridine DNA labelling reveals host and parasite proliferation in a fish-myxozoan model

AutorEstensoro, Itziar ; Pérez-Cordón, Gregorio; Sitjà-Bobadilla, Ariadna ; Piazzon de Haro, María Carla
Palabras claveMyxozoa
Immunohistochemistry
Intestine
PCNA
Sparus aurata
Fecha de publicaciónabr-2018
CitaciónJournal of Fish Diseases
ResumenEnteromyxum leei is a myxozoan parasite responsible for enteritis in gilthead sea bream (Sparus aurata). The parasite proliferates in the paracellular space of the intestinal epithelium and induces an inflammatory reaction. In order to assess intestinal cell turnover and parasite proliferation, fish were infected with the parasite by anal intubation and after 17 and 64 days, the cell proliferative marker bromodeoxyuridine (BrdU) was administered and, after 24 hours, tissue samples were taken for immunohistochemical detection. Parasite exposure induced increased epithelial and immune cell proliferation in all intestinal segments at all time points, even before parasite establishment. This increased turnover was triggered early after intubation and mainly at a local level, as shown by an increased proliferating cell nuclear antigen (pcna) gene expression only at the posterior intestine after 17 days (not found in lymphohaematopietic organs). Incorporation of BrdU in parasite secondary and tertiary daughter cells indicated that parasite endogeny is not by schizogonial division, which uses de novo synthesis pathway of pyrimidines. Altogether, BrdU immunolabelling and pcna gene expression showed the rapid proliferative response of the fish intestines upon a myxozoan infection and how this response is effectively triggered even before the parasite reaches or establishes in the site.
Versión del editor10.1111/jfd.12765
URIhttp://hdl.handle.net/10261/163279
DOI10.1111/jfd.12765
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