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dc.contributor.authorJiménez-Fernández, Alicia-
dc.contributor.authorLópez-Sánchez, Aroa-
dc.contributor.authorJiménez-Díaz, Lorena-
dc.contributor.authorNavarrete, Blanca-
dc.contributor.authorCalero, Patricia-
dc.contributor.authorPlatero, Ana Isabel-
dc.contributor.authorGovantes, Fernando-
dc.date.accessioned2018-04-06T10:13:54Z-
dc.date.available2018-04-06T10:13:54Z-
dc.date.issued2016-
dc.identifierdoi: 10.1371/journal.pone.0163142-
dc.identifiere-issn: 1932-6203-
dc.identifier.citationPLoS ONE 11(9): e0163142 (2016)-
dc.identifier.urihttp://hdl.handle.net/10261/163247-
dc.description.abstractMost bacteria alternate between a free living planktonic lifestyle and the formation of structured surface-Associated communities named biofilms. The transition between these two lifestyles requires a precise and timely regulation of the factors involved in each of the stages that has been likened to a developmental process. Here we characterize the involvement of the transcriptional regulator FleQ and the second messenger cyclic diguanylate in the coordinate regulation of multiple functions related to motility and surface colonization in Pseudomonas putida. Disruption of fleQ caused strong defects in flagellar motility, biofilm formation and surface attachment, and the ability of this mutation to suppress multiple biofilm-related phenotypes associated to cyclic diguanylate overproduction suggests that FleQ mediates cyclic diguanylate signaling critical to biofilm growth. We have constructed a library containing 94 promoters potentially involved in motility and biofilm development fused to gfp and lacZ, screened this library for FleQ and cyclic diguanylate regulation, and assessed the involvement of alternative σ factors σN and FliA in the transcription of FleQregulated promoters. Our results suggest a dual mode of action for FleQ. Low cyclic diguanylate levels favor FleQ interaction with σN-dependent promoters to activate the flagellar cascade, encompassing the flagellar cluster and additional genes involved in cyclic diguanylate metabolism, signal transduction and gene regulation. On the other hand, characterization of the FleQ-regulated σN-and FliA-independent PlapA and PbcsD promoters revealed two disparate regulatory mechanisms leading to a similar outcome: The synthesis of biofilm matrix components in response to increased cyclic diguanylate levels.-
dc.description.sponsorshipThis work was supported by Ministerio de Ciencia e Innovación, Spain (http://www.idi.mineco.gob.es/) and European Regional Development fund (http://ec.europa.eu/regional_policy/en/funding/erdf/), Grant BIO2010-17853, awarded to FG; Ministerio de Economía y Competitividad, Spain (http://www.idi.mineco.gob.es/) and European Regional Development fund (http://ec.europa.eu/regional_policy/en/funding/erdf/), Grant BIO2013-420173-P, awarded to FG; and Consejo Superior de Investigaciones Científicas, Spain (http://www.csic.es/), JAE-Predoc 2010 scholarship, awarded to AJ-F.-
dc.publisherPublic Library of Science-
dc.relationinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-42073-P-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleComplex interplay between FleQ, cyclic diguanylate and multiple σ factors coordinately regulates flagellar motility and biofilm development in Pseudomonas putida-
dc.typeartículo-
dc.identifier.doi10.1371/journal.pone.0163142-
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0163142-
dc.date.updated2018-04-06T10:13:54Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.pmid27636892-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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