English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163247
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Complex interplay between FleQ, cyclic diguanylate and multiple σ factors coordinately regulates flagellar motility and biofilm development in Pseudomonas putida

AuthorsJiménez-Fernández, Alicia ; López-Sánchez, Aroa ; Jiménez-Díaz, Lorena; Navarrete, Blanca; Calero, Patricia ; Platero, Ana Isabel ; Govantes, Fernando
Issue Date2016
PublisherPublic Library of Science
CitationPLoS ONE 11(9): e0163142 (2016)
AbstractMost bacteria alternate between a free living planktonic lifestyle and the formation of structured surface-Associated communities named biofilms. The transition between these two lifestyles requires a precise and timely regulation of the factors involved in each of the stages that has been likened to a developmental process. Here we characterize the involvement of the transcriptional regulator FleQ and the second messenger cyclic diguanylate in the coordinate regulation of multiple functions related to motility and surface colonization in Pseudomonas putida. Disruption of fleQ caused strong defects in flagellar motility, biofilm formation and surface attachment, and the ability of this mutation to suppress multiple biofilm-related phenotypes associated to cyclic diguanylate overproduction suggests that FleQ mediates cyclic diguanylate signaling critical to biofilm growth. We have constructed a library containing 94 promoters potentially involved in motility and biofilm development fused to gfp and lacZ, screened this library for FleQ and cyclic diguanylate regulation, and assessed the involvement of alternative σ factors σN and FliA in the transcription of FleQregulated promoters. Our results suggest a dual mode of action for FleQ. Low cyclic diguanylate levels favor FleQ interaction with σN-dependent promoters to activate the flagellar cascade, encompassing the flagellar cluster and additional genes involved in cyclic diguanylate metabolism, signal transduction and gene regulation. On the other hand, characterization of the FleQ-regulated σN-and FliA-independent PlapA and PbcsD promoters revealed two disparate regulatory mechanisms leading to a similar outcome: The synthesis of biofilm matrix components in response to increased cyclic diguanylate levels.
Publisher version (URL)https://doi.org/10.1371/journal.pone.0163142
URIhttp://hdl.handle.net/10261/163247
Identifiersdoi: 10.1371/journal.pone.0163142
e-issn: 1932-6203
Appears in Collections:(CABD) Artículos
Files in This Item:
File Description SizeFormat 
complexputida.PDF2,68 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.