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Título : A role for SNAP25 in internalization of kainate receptors and synaptic plasticity
Otros títulos: SNAP25 controls KAR endocytosis
Autor : Selak, Sanja, Paternain, Ana V., Aller, María Isabel, Picó, Esther, Rivera, Rocío, Lerma Gómez, Juan
Palabras clave : Kainate receptors (KARs)
Synaptic strength
Synaptic plasticity
SNAP25
GluK5 subunits
Protein complexes
Signaling
Fecha de publicación : 13-Aug-2009
Editor: Elsevier
Citación : Neuron 63(3): 357-371 (2009)
Resumen: Regulation of surface insertion and internalization of AMPA and NMDA receptors has emerged as a key mechanism for the control of synaptic strength. Regulatory elements for synaptic kainate receptors (KARs) are, however, largely undetermined. We have found that SNAP25 is critical for the synaptic removal of KARs, acting via GluK5 (i.e., KA2) subunits. SNAP25 coimmunoprecipitates with protein complexes containing PICK1, GRIP1, and GluK5 and colocalizes with GluK5 in both hippocampal neurons and transfected HEK293 cells. In hippocampal slices, purified SNAP25 antibodies and blocking peptides caused a GluK5-dependent run-up of KARs-mediated EPSC (EPSC-KAR) recorded from CA3 pyramidal neurons when included in the patch pipette and prevented activity-dependent long-term depression of EPSC-KAR. As EPSC-KAR LTD, SNAP25/PICK1/GluK5 interactions are dynamically regulated by PKC.
Descripción : 15 pages, 9 figures.-- PMID: 19679075 [PubMed].-- Supporting information including detailed Experimental Procedures and eight figures available at: http://dx.doi.org/10.1016/j.neuron.2009.07.017
Versión del editor: http://dx.doi.org/10.1016/j.neuron.2009.07.017
URI : http://hdl.handle.net/10261/16278
ISSN: 0896-6273
DOI: 10.1016/j.neuron.2009.07.017
Citación : Neuron 63(3): 357-371 (2009)
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