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dc.contributor.authorCasa Villegas, Maryes_ES
dc.contributor.authorMarín Navarro, Juliaes_ES
dc.contributor.authorPolaina Molina, Julioes_ES
dc.date.accessioned2018-03-14T08:38:15Z-
dc.date.available2018-03-14T08:38:15Z-
dc.date.issued2017-11-16-
dc.identifier.citationACS Omega 2 (11): 8062–8068 (2017)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/162204-
dc.description.abstractThe α-glucosidase encoded by the aglA gene of Aspergillus niger is a secreted enzyme belonging to family 31 of glycoside hydrolases. This enzyme has a retaining mechanism of action and displays transglycosylating activity that makes it amenable to be used for the synthesis of isomaltooligosaccharides (IMOs). We have expressed the aglA gene in Saccharomyces cerevisiae under control of a galactose-inducible promoter. Recombinant yeast cells expressing the aglA gene produced extracellular α-glucosidase activity about half of which appeared cell bound whereas the other half was released into the culture medium. With maltose as the substrate, panose is the main transglycosylation product after 8 h of incubation, whereas isomaltose is predominant after 24 h. Isomaltose also becomes predominant at shorter times if a mixture of maltose and glucose is used instead of maltose. To facilitate IMO production, we have designed a procedure by which yeast cells can be used directly as the catalytic agent. For this purpose, we expressed in S. cerevisiae gene constructs in which the aglA gene is fused to glycosylphosphatidylinositol anchor sequences, from the yeast SED1 gene, that determine the covalent binding of the hybrid protein to the cell membrane. The resulting hybrid enzymes were stably attached to the cell surface. The cells from cultures of recombinant yeast strains expressing aglA-SED1 constructions can be used to produce IMOs in successive batches.es_ES
dc.description.sponsorshipThis work was funded by grants AGL2016-75245-R and BIO2013-48779-C4-3-R from Spain’s “Secretaría de Estado de Investigación, Desarrollo e Innovación”. M.C.-V. was supported by a SENESCYT predoctoral fellowship from the Government of República del Ecuador.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.relationMINECO/ICTI2013-2016/AGL2016-75245-Res_ES
dc.relationMINECO/ICTI2013-2016/BIO2013-48779-C4-3-Res_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectCarbohydrateses_ES
dc.subjectGeneticses_ES
dc.subjectPhysical and chemical processeses_ES
dc.subjectProteinses_ES
dc.titleSynthesis of Isomaltooligosaccharides by Saccharomyces cerevisiae Cells Expressing Aspergillus niger α-Glucosidasees_ES
dc.typeartículoes_ES
dc.identifier.doi10.1021/acsomega.7b01189-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1021/acsomega.7b01189es_ES
dc.identifier.e-issn2470-1343-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderSecretaría de Educación Superior, Ciencia y Tecnología (Ecuador)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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