Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/161677
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Pérez-Lago, Laura | es_ES |
dc.contributor.author | Herranz, Marta | es_ES |
dc.contributor.author | Comas, Iñaki | es_ES |
dc.contributor.author | Sierra, Olalla | es_ES |
dc.contributor.author | Bouza, Emilio | es_ES |
dc.contributor.author | García de Viedma, Darío | es_ES |
dc.date.accessioned | 2018-03-06T09:34:25Z | - |
dc.date.available | 2018-03-06T09:34:25Z | - |
dc.date.issued | 2016-10-13 | - |
dc.identifier.citation | Clinical Microbiology and Infection 23(2): 92-97 (2017) | es_ES |
dc.identifier.issn | 1198-743X | - |
dc.identifier.uri | http://hdl.handle.net/10261/161677 | - |
dc.description | et al. | es_ES |
dc.description.abstract | [Objective]: Molecular epidemiology techniques in tuberculosis (TB) can identify high-risk strains that are actively transmitted. We aimed to implement a novel strategy to optimize the identification and control of multidrug-resistant (MDR) TB in a specific population. [Methods]: We developed a strain-specific PCR tailored from whole genome sequencing (WGS) data to track a specific MDR prevalent strain in Equatorial Guinea (EG-MDR). [Results]: The PCR was applied prospectively on remnants of GeneXpert reaction mixtures owing to the lack of culture facilities in Equatorial Guinea. In 147 (93%) of 158 cases, we were able to differentiate between infection by the EG-MDR strain or by any other strain and found that 44% of all rifampicin-resistant TB cases were infected by EG-MDR. We also analysed 93 isolates obtained from Equatorial Guinea 15 years ago, before MDR-TB had become the problem it is today. We found that two of the scarce historical MDR cases were infected by EG-MDR. WGS revealed low variability-six single nucleotide polymorphisms acquired by this strain over 15 years-likely because of the lack in the country of a specific program to treat MDR-TB. [Conclusions]: Our novel strategy, which integrated WGS analysis and strain-specific PCRs, represents a low-cost, rapid and transferable strategy that allowed a prospective efficient survey and fast historical analysis of MDR-TB in a population. | es_ES |
dc.description.sponsorship | This study was funded by Plan Estatal I+D+I 2013-2016, ISCIII (13/01207; 15/01554) and cofunded by the Fondo Europeo de Desarrollo Regional (FEDER): “Una manera de hacer Europa”. We also received funding from Fondation Merieux (DM/CL/cb15) and a Grant for International Cooperation Projects from the IiSGM (I and II-COOP-INT 2015). L.P. holds a Miguel Servet research grant (CP15/00075), MINECO research grant (SAF2013-43521-R); I.C. holds a European Research Council (ERC) grant (638553-TB-ACCELERATE). Funding was also received from the Spanish Network for Research in Infectious Diseases (REIPI, RD12/0015 to GT). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-43521-R | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/638553 | es_ES |
dc.relation.isversionof | Postprint | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Tuberculosis | es_ES |
dc.subject | MDR | es_ES |
dc.subject | Equatorial Guinea | es_ES |
dc.subject | Screening | es_ES |
dc.subject | Emergence | es_ES |
dc.title | A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.cmi.2016.10.006 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.cmi.2016.10.006 | es_ES |
dc.identifier.e-issn | 1469-0691 | - |
dc.rights.license | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.contributor.funder | European Research Council | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000781 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
Aparece en las colecciones: | (IBV) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2017 Clin Microbiol Infect_vers_Aut.pdf | 135,88 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
12
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
11
checked on 23-feb-2024
Page view(s)
324
checked on 23-abr-2024
Download(s)
230
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons