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Title

A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence

AuthorsPérez-Lago, Laura; Herranz, Marta; Comas, Iñaki ; Sierra, Olalla; Bouza, Emilio; García de Viedma, Darío
KeywordsTuberculosis
MDR
Equatorial Guinea
Screening
Emergence
Issue Date13-Oct-2016
PublisherElsevier
CitationClinical Microbiology and Infection 23(2): 92-97 (2017)
Abstract[Objective]: Molecular epidemiology techniques in tuberculosis (TB) can identify high-risk strains that are actively transmitted. We aimed to implement a novel strategy to optimize the identification and control of multidrug-resistant (MDR) TB in a specific population. [Methods]: We developed a strain-specific PCR tailored from whole genome sequencing (WGS) data to track a specific MDR prevalent strain in Equatorial Guinea (EG-MDR). [Results]: The PCR was applied prospectively on remnants of GeneXpert reaction mixtures owing to the lack of culture facilities in Equatorial Guinea. In 147 (93%) of 158 cases, we were able to differentiate between infection by the EG-MDR strain or by any other strain and found that 44% of all rifampicin-resistant TB cases were infected by EG-MDR. We also analysed 93 isolates obtained from Equatorial Guinea 15 years ago, before MDR-TB had become the problem it is today. We found that two of the scarce historical MDR cases were infected by EG-MDR. WGS revealed low variability-six single nucleotide polymorphisms acquired by this strain over 15 years-likely because of the lack in the country of a specific program to treat MDR-TB. [Conclusions]: Our novel strategy, which integrated WGS analysis and strain-specific PCRs, represents a low-cost, rapid and transferable strategy that allowed a prospective efficient survey and fast historical analysis of MDR-TB in a population.
Descriptionet al.
Publisher version (URL)http://dx.doi.org/10.1016/j.cmi.2016.10.006
URIhttp://hdl.handle.net/10261/161677
DOI10.1016/j.cmi.2016.10.006
ISSN1198-743X
E-ISSN1469-0691
Appears in Collections:(IBV) Artículos
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