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Closed Access item Number and phylogenetic affiliation of bacteria assimilating dimethylsulfoniopropionate and leucine in the ice-covered coastal Arctic Ocean

Authors:Vila-Costa, Maria
Simó, Rafel
Alonso-Sáez, Laura
Pedrós-Alió, Carlos
Keywords:Bacteria, DMSP, leucine, microautoradiography, FISH, Beaufort Sea, Franklin Bay, Arctic winter
Issue Date:1-Dec-2008
Citation:Journal of Marine Systems 74(3-4): 957-963 (2008)
Abstract:The ability of bacteria to assimilate sulfur from dimethylsulfoniopropionate (DMSP) was examined in the western Arctic Ocean by combining microautoradiography and fluorescence in situ hybridization (FISH). Assimilation of leucine was also measured for comparative purposes since leucine is considered a universal substrate for bacteria, which use it for protein synthesis. Samples were collected at 3 m depth, through a hole in the ice, in the CASES (Canadian Arctic Shelf Ecosystem Study) overwintering station in Franklin Bay (eastern Beaufort Sea) in March and May 2004 to compare two contrasting situations: winter and early spring. FISH counts indicated that the bacterial assemblage consisted of α- (up to 60% of the EUB positive cells), β- (up to 10%) and γ-proteobacteria (around 20%), and Bacteroidetes (up to 60%). The β-proteobacteria were not active with any of the two substrates tested. The remaining groups were much less efficient at assimilating DMSP-sulfur (5% of the cells) than leucine (20–35%) both in winter and in spring. Only the Roseobacter group of α-proteobacteria showed a similar assimilation of both substrates.
Description:7 pages, 5 figures, 1 table.-- Available online Oct 24, 2007.-- Issue title: "Sea ice and life in a river-influenced arctic shelf ecosystem".
Full-text version available Open Acces at: http://www.icm.csic.es/bio/projects/icmicrobis/pdf
Publisher version (URL):http://dx.doi.org/10.1016/j.jmarsys.2007.10.006
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