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dc.contributor.authorCanales, Ángeleses_ES
dc.contributor.authorRösinger, Marceles_ES
dc.contributor.authorSastre, Javieres_ES
dc.contributor.authorFelli, Isabella C.es_ES
dc.contributor.authorJiménez-Barbero, Jesúses_ES
dc.contributor.authorGiménez-Gallego, Guillermoes_ES
dc.contributor.authorFernández-Tornero, Carloses_ES
dc.date.accessioned2018-02-22T09:53:46Z-
dc.date.available2018-02-22T09:53:46Z-
dc.date.issued2017-12-06-
dc.identifier.citationPLoS ONE 12(12): e0189171 (2017)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/161177-
dc.description15 p.-6 fig.es_ES
dc.description.abstractC/EBP-homologous protein (CHOP) is a key determinant of the apoptotic response to endoplasmic reticulum stress or DNA damage. As a member of the C/EBP family, CHOP contains a low complexity N-terminal region involved in transcriptional activation, followed by a bZIP that binds DNA after dimerization. However, in contrast to other C/EBPs, CHOP directs binding to non-canonical C/EBP sites due to unique substitutions in its DNA-binding domain. Herein, we show that the N-terminal region of CHOP is intrinsically unstructured but contains two segments presenting α-helical propensity. One of these segments is conserved in other C/EBPs and mediates essential roles of CHOP, including regulation through phosphorylation. The second segment is placed within a proteolytic-resistant portion of the protein and exhibits reduced flexibility. Moreover, the DNA-binding region of CHOP also contains a segment with α-helical character towards its most N-terminal part. Our results suggest that structure-prone segments scattered within disordered regions may be critical for macromolecular recognition during CHOP-mediated transcriptional activation.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Economy and Competitiveness [BFU2013-48374-P to C.F.-T., CTQ2016-76263-P to A.C.]; the Ramón Areces Foundation [to C.F.-T.]; and the European Commission [BioNMR (Contract No. 261863) to I.C.F.].es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.relationMINECO/ICTI2013-2016/BFU2013-48374-Pes_ES
dc.relationMINECO/ICTI2013-2016/CTQ2016-76263-Pes_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/261863es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titleHidden α-helical propensity segments within disordered regions of the transcriptional activator CHOPes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1371/journal.pone.0189171-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0189171es_ES
dc.identifier.e-issn1932-6203-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderFundación Ramón Areceses_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100008054es_ES
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