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Revealing a polyamine-ethylene regulatory node linked to drought tolerance/susceptibility in oat

AuthorsMontilla-Bascón, Gracia ; Canales, Francisco José; Mur, Luis A. J.; Prats, Elena
Stress resistance
Nitric oxide
Issue DateJul-2016
Citation10th International Oat Conference (2016)
AbstractUnderstanding plant tolerance to drought is of fundamental importance in facilitating the breeding of drought tolerant plants. However drought tolerance responses are extremely complex and involve a wide range of processes and intricate signaling events. In a previous work we monitored endogenous nitric oxide (NO) production in drought susceptible and resistant oat cultivars during drought and data suggested lesser production in the resistant genotype. This was conñnned by using transgenic barley lines overexpressing the barley non-symbiotic hemoglobin gene HvHbl which oxidizes NOto N03-. Investigation ofpolyamine metabolism in wild type and HvHbl transgenic background suggested that NO and ethylene were components in a subtle reprogramrning ofpolyamine biosynthetic gene-expression and associated post-transcriptional modification which contribute to either drought tolerance or susceptibility. We are now performing further studies to dissect the role of ethylene and its crosstalk with polyamines during drought resistance in oats. To this aim we are using the oat cultivars Patones and Flega, characterized as drought tolerant and susceptible, respectively. Gene expression analysis through RT-PCR are being carried out to determine the expression of specific genes for polyamine and ethylene pathway, i.e. arginine decarboxylase (ADC), and 1-aminocyclopropane-1-carboxylate oxidase (ACO), respectively, and also for genes that are common nodes for polyamine and ethylene biosynthesis: i.e. methionine adenosyltransferase (MAT), S-adenosylmethionine decarboxylase (AdoMetDC) and 1-aminocyclopropane-1-carboxylate [ACC] synthase (ACS), which catalyzes the first committed step in ethylene biosynthesis diverging from polyamine pathway. Preliminary results showed an increase of more than 20 fold of ACO gene expression in cultivar Flega under drought compared with Patones suggesting an increase of ethylene biosynthesis linked with drought susceptibility. Further work will include the monitoring of in vivo ethylene production in Flega and Patones under well-watered and drought conditions.
DescriptionTrabajo presentado en la 10th International Oat Conference, celebrada San Petersburgo del 11 al 15 de julio de 2016.
Appears in Collections:(IAS) Comunicaciones congresos
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