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Título: | Rapid on-chip apoptosis assay on human carcinoma cells based on annexin-V/quantum dot probes |
Autor: | Montón, Helena CSIC; Medina-Sánchez, Mariana CSIC; Soler, Joan; Chalupniak, Andrzej CSIC; Nogués, Carme; Merkoçi, Arben CSIC ORCID | Palabras clave: | Optical detection Quantum dots Drug screening Modular microfluidics Apoptosis |
Fecha de publicación: | 2017 | Editor: | Elsevier | Citación: | Biosensors and Bioelectronics 94: 408-414 (2017) | Resumen: | Despite all the efforts made over years to study the cancer expression and the metastasis event, there is not a clear understanding of its origins and effective treatment. Therefore, more specialized and rapid techniques are required for studying cell behaviour under different drug-based treatments. Here we present a quantum dot signalling-based cell assay carried out in a segmental microfluidic device that allows studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine translocation that occurs in early apoptosis. The developed platform combines the automatic generation of a drug gradient concentration, allowing exposure of cancer cells to different doses, and the immunolabeling of the apoptotic cells using quantum dot reporters. Thereby a complete cell-based assay for efficient drug screening is performed showing a clear correlation between drug dose and amount of cells undergoing apoptosis. | URI: | http://hdl.handle.net/10261/160232 | DOI: | 10.1016/j.bios.2017.03.034 | Identificadores: | doi: 10.1016/j.bios.2017.03.034 issn: 0956-5663 e-issn: 1873-4235 |
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