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dc.contributor.authorDomínguez-Moñino, Irenees_ES
dc.contributor.authorJurado, Valmees_ES
dc.contributor.authorGonzález Pimentel, José L.es_ES
dc.contributor.authorMiller, A. Z.es_ES
dc.contributor.authorHermosín, Bernardoes_ES
dc.contributor.authorSáiz-Jiménez, Cesáreoes_ES
dc.date.accessioned2018-02-05T07:58:52Z-
dc.date.available2018-02-05T07:58:52Z-
dc.date.issued2018-05-
dc.identifier.citationSystematic and Applied Microbiology 41(3):167-172 (2018)es_ES
dc.identifier.issn0723-2020-
dc.identifier.urihttp://hdl.handle.net/10261/160092-
dc.description19 páginas.-- 2 figuras.-- 2 tablas.-- 43 referenciases_ES
dc.description.abstractTwo Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain. Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%). DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively. Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi. Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47. °C (optimum 37. °C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%). In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G + C content was 38. mol%. On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp. nov. is proposed. The type strain is 0911MAR22V3T (=LMG 27963T = CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.es_ES
dc.description.sponsorshipThis study was supported by the project RNM 5137, Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, co-financed by 2007-2013 FEDER programmees_ES
dc.language.isospaes_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectBacilluses_ES
dc.subjectBacillus onubensises_ES
dc.subjectShow cavees_ES
dc.subject16S rRNA gene sequencees_ES
dc.subjectPolyphasic taxonomyes_ES
dc.titleBacillus onubensis sp. nov., isolated from the air of two Andalusian caveses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.syapm.2018.01.001-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.syapm.2018.01.001es_ES
dc.identifier.e-issn1618-0984-
dc.embargo.terms2019-05-01es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.contributor.funderJunta de Andalucíaes_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.contributor.orcidJurado, Valme [0000-0003-0972-9909]-
dc.contributor.orcidSáiz-Jiménez, Cesáreo [0000-0003-0036-670X]-
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