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Proteomic characterization of transcription and splicing factors associated with a metastatic phenotype in colorectal cancer

Other TitlesTranscription factors in metastatic colorectal cancer [Running title]
AuthorsTorres, Sofía ; García-Palmero, Irene ; Marín-Vicente, Consuelo; Bartolomé, Rubén Álvaro ; Calviño, Eva ; Fernandez-Aceñero, M. Jesús; Casal, J. Ignacio
Transcription factors
Splicing factors
Colorectal cancer
Issue Date5-Jan-2018
PublisherAmerican Chemical Society
CitationJ Proteome Res 17(1):252-264 (2018)
AbstractWe investigated new transcription and splicing factors associated with the metastatic phenotype in colorectal cancer. A concatenated tandem array of consensus transcription factor (TF)-response elements was used to pull down nuclear extracts in two different pairs of colorectal cancer cells, KM12SM/KM12C and SW620/480, genetically related but differing in metastatic ability. Proteins were analyzed by label-free LC-MS and quantified with MaxLFQ. We found 240 proteins showing a significant dysregulation in highly metastatic KM12SM cells relative to nonmetastatic KM12C cells and 257 proteins in metastatic SW620 versus SW480. In both cell lines there were similar alterations in genuine TFs and components of the splicing machinery like UPF1, TCF7L2/TCF-4, YBX1, or SRSF3. However, a significant number of alterations were cell-line specific. Functional silencing of MAFG, TFE3, TCF7L2/TCF-4, and SRSF3 in KM12 cells caused alterations in adhesion, survival, proliferation, migration, and liver homing, supporting their role in metastasis. Finally, we investigated the prognostic value of the altered TFs and splicing factors in cancer patients. SRSF3 and SFPQ showed significant prognostic value. We observed that SRSF3 displayed a gradual loss of expression associated with cancer progression. Loss of SRSF3 expression was significantly associated with poor survival and shorter disease-free survival, particularly in early stages, in colorectal cancer.
Description44 p.-7 fig.
Publisher version (URL)http://dx.doi.org/10.1021/acs.jproteome.7b00548
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