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dc.contributor.authorBárány, Ivettes_ES
dc.contributor.authorBerenguer, Eduardoes_ES
dc.contributor.authorSolís, María Teresaes_ES
dc.contributor.authorPérez-Pérez, Yolandaes_ES
dc.contributor.authorSantamaría, María Estrellaes_ES
dc.contributor.authorCrespo, José L.es_ES
dc.contributor.authorRisueño, María Carmenes_ES
dc.contributor.authorDíaz, Isabeles_ES
dc.contributor.authorTestillano, P.S.es_ES
dc.date.accessioned2018-01-09T12:46:12Z-
dc.date.available2018-01-09T12:46:12Z-
dc.date.issued2018-01-04-
dc.identifier.citationJ. Exp. Bot.69(6):1387-1402 (2018)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/158937-
dc.description16 p.-8 fig.-1 tab.-1 tab. supl.- 1 mat. supl.es_ES
dc.description.abstractMicrospores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators.es_ES
dc.description.sponsorshipThis work is supported by projects AGL2014-52028-R and AGL2017-82447-R funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (ERDF/FEDER). YPP is the recipient of a grant (PEJ15/BIO/AI-01S8) funded by Comunidad de Madrid and European Commission through ERDF/FEDER.es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.relationMINECO/ICTI2013-2016/AGL2014-52028-Res_ES
dc.relationMINECO/ICTI2013-2016/AGL2017-82447-Res_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectAutophagyes_ES
dc.subjectBarleyes_ES
dc.subjectCaspase-like activityes_ES
dc.subjectBathepsinses_ES
dc.subjectCell deathes_ES
dc.subjectCysteine C1A proteaseses_ES
dc.subjectMicrospore embryogenesises_ES
dc.subjectROSes_ES
dc.subjectStress responsees_ES
dc.titleAutophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barleyes_ES
dc.typeArtículoes_ES
dc.identifier.doi10.1093/jxb/erx455-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://doi.org/10.1093/jxb/erx455es_ES
dc.identifier.e-issn1460-2431-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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