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Title

Identification, cloning, and expression analysis of three phytoene synthase genes from Cucurbita pepo

AuthorsObrero, Ángeles; González-Verdejo, Clara Isabel; Román, Belén; Gómez Jiménez de Cisneros, Pedro; Die, José V. ; Ampomah-Dwamena, C.
KeywordsFruit development
Phylogenetic tree
Summer squash
Transcriptional analysis
Issue DateJun-2015
PublisherSpringer
CitationBiologia Plantarum 59(2): 201-210 (2015)
AbstractAn essential step in the carotenoid biosynthesis pathway is the formation of phytoene by phytoene synthase (PSY). In this study, three new genes coding PSYs (CpPSYA, CpPSYB, and CpPSYC) were cloned from Cucurbita pepo and their expression patterns analysed in three cultivars of summer squash which had a different carotenoid content. The gene sequences had a high similarity with those from other plant species, and their predicted proteins were significantly different from each other. A phylogenetic analysis indicates that CpPSYA and CpPSYB shared a high homology and were also homologous with PSYs from others cucurbits, whereas CpPSYC was more closely related to orthologues from strawberry and carrot. An expression analysis revealed that CpPSYA had a higher expression in flowers compared to leaves and showed a differential expression during fruit development. The amount of CpPSYA transcript was higher in fruits with a higher carotenoid content than in those with a lower carotenoid content. However, CpPSYB and CpPSYC showed a relatively high expression in leaves, and their expression in fruits varied among the different cultivars and fruit tissues. These results suggest that the CpPSY genes were under different regulatory mechanisms and they may have different roles in C. pepo.
Publisher version (URL)http://doi.org/10.1007/s10535-015-0504-3
URIhttp://hdl.handle.net/10261/158798
DOI10.1007/s10535-015-0504-3
ISSN0006-3134
E-ISSN1573-8264
Appears in Collections:(IAS) Artículos
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