Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/158479
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish |
Autor: | Suárez-Bregua, Paula CSIC ORCID; Chien, Chien-ju; Megias, Manuel; Du, Shao Jun; Rotllant, Josep CSIC ORCID | Palabras clave: | Transgenic Zebrafish Skeletal muscle Cardiac muscle Muscle-specificity Myod Mef2 |
Fecha de publicación: | 2017 | Editor: | John Wiley & Sons | Citación: | Developmental Dynamics 246(12): 992-1000 (2017) | Resumen: | Background: Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in Zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown. Results: The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter Zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter Zebrafish lines generated replicate the endogenous mustn1b expression pattern in early Zebrafish embryos. Specific site directed-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions, each one corresponding to a specific functional transcription factor binding site. Conclusions: Our data indicate that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2-kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factors | Descripción: | 9 pages, 1 table, 6 figures | Versión del editor: | http://dx.doi.org/10.1002/dvdy.24591 | URI: | http://hdl.handle.net/10261/158479 | DOI: | 10.1002/dvdy.24591 | ISSN: | 1058-8388 | E-ISSN: | 1097-0177 |
Aparece en las colecciones: | (IIM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Promoter_architecture_2017.pdf | 869,08 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
6
checked on 04-mar-2024
WEB OF SCIENCETM
Citations
5
checked on 24-feb-2024
Page view(s)
311
checked on 28-mar-2024
Download(s)
217
checked on 28-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.