Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/158479
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish

AutorSuárez-Bregua, Paula CSIC ORCID; Chien, Chien-ju; Megias, Manuel; Du, Shao Jun; Rotllant, Josep CSIC ORCID
Palabras claveTransgenic Zebrafish
Skeletal muscle
Cardiac muscle
Muscle-specificity
Myod
Mef2
Fecha de publicación2017
EditorJohn Wiley & Sons
CitaciónDevelopmental Dynamics 246(12): 992-1000 (2017)
ResumenBackground: Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in Zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown. Results: The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter Zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter Zebrafish lines generated replicate the endogenous mustn1b expression pattern in early Zebrafish embryos. Specific site directed-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions, each one corresponding to a specific functional transcription factor binding site. Conclusions: Our data indicate that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2-kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factors
Descripción9 pages, 1 table, 6 figures
Versión del editorhttp://dx.doi.org/10.1002/dvdy.24591
URIhttp://hdl.handle.net/10261/158479
DOI10.1002/dvdy.24591
ISSN1058-8388
E-ISSN1097-0177
Aparece en las colecciones: (IIM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Promoter_architecture_2017.pdf869,08 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

6
checked on 04-mar-2024

WEB OF SCIENCETM
Citations

5
checked on 24-feb-2024

Page view(s)

311
checked on 28-mar-2024

Download(s)

217
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.