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dc.contributor.authorSuárez-Bregua, Paulaes_ES
dc.contributor.authorChien, Chien-jues_ES
dc.contributor.authorMegias, Manueles_ES
dc.contributor.authorDu, Shao Junes_ES
dc.contributor.authorRotllant, Josepes_ES
dc.date.accessioned2017-12-22T09:41:40Z-
dc.date.available2017-12-22T09:41:40Z-
dc.date.issued2017-
dc.identifier.citationDevelopmental Dynamics 246(12): 992-1000 (2017)es_ES
dc.identifier.issn1058-8388-
dc.identifier.urihttp://hdl.handle.net/10261/158479-
dc.description9 pages, 1 table, 6 figureses_ES
dc.description.abstractBackground: Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in Zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown. Results: The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter Zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter Zebrafish lines generated replicate the endogenous mustn1b expression pattern in early Zebrafish embryos. Specific site directed-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions, each one corresponding to a specific functional transcription factor binding site. Conclusions: Our data indicate that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2-kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factorses_ES
dc.description.sponsorshipThis work was funded by the Spanish Science and Innovation Ministry grants ALG2011-23581 and AGL2014-52473R (to J.R.). P. Suarez-Bregua was supported by a Campus do Mar PhD grant, Xunta de Galicia.es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.relationMINECO/ICTI2013-2016/AGL2014-52473-Res_ES
dc.relation.isversionofPreprintes_ES
dc.rightsopenAccessen_EN
dc.subjectTransgenic Zebrafishes_ES
dc.subjectSkeletal musclees_ES
dc.subjectCardiac musclees_ES
dc.subjectMuscle-specificityes_ES
dc.subjectMyodes_ES
dc.subjectMef2es_ES
dc.titlePromoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafishes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1002/dvdy.24591-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1002/dvdy.24591es_ES
dc.identifier.e-issn1097-0177-
dc.embargo.terms2018-12-01es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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