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Title

Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish

AuthorsSuárez-Bregua, Paula ; Chien, Chien-ju; Megias, Manuel; Du, Shao Jun; Rotllant, Josep
KeywordsTransgenic Zebrafish
Skeletal muscle
Cardiac muscle
Muscle-specificity
Myod
Mef2
Issue Date2017
PublisherJohn Wiley & Sons
CitationDevelopmental Dynamics 246(12): 992-1000 (2017)
AbstractBackground: Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in Zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown. Results: The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter Zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter Zebrafish lines generated replicate the endogenous mustn1b expression pattern in early Zebrafish embryos. Specific site directed-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions, each one corresponding to a specific functional transcription factor binding site. Conclusions: Our data indicate that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2-kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factors
Description9 pages, 1 table, 6 figures
Publisher version (URL)http://dx.doi.org/10.1002/dvdy.24591
URIhttp://hdl.handle.net/10261/158479
DOI10.1002/dvdy.24591
ISSN1058-8388
E-ISSN1097-0177
Appears in Collections:(IIM) Artículos
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